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Staining and fluorescent tagging can serve similar purposes. Biological staining is also used to mark cells in flow cytometry, and to flag proteins or nucleic acids in gel electrophoresis. Light microscopes are used for viewing stained samples at high magnification, typically using bright-field or epi-fluorescence illumination.
The Biological Stain Commission (BSC) is an organization that provides third-party testing and certification of dyes and a few other compounds that are used to enhance contrast in specimens examined in biological and medical laboratories.
Blood film stained with Giemsa showing Plasmodium (center of image), the parasite that causes malaria infections.. In 1891 Romanowsky [8] [9] [10] developed a stain using a mixture of eosin (typically eosin Y) and aged solutions of methylene blue that formed hues unattributable to the staining components alone: distinctive shades of purple in the chromatin of the cell nucleus and within ...
Biological tissue has little inherent contrast in either the light or electron microscope. [17] Staining is employed to give both contrast to the tissue as well as highlighting particular features of interest. When the stain is used to target a specific chemical component of the tissue (and not the general structure), the term histochemistry is ...
The H&E staining procedure is the principal stain in histology [3] [7] [2] [5] in part because it can be done quickly, [7] is not expensive, and stains tissues in such a way that a considerable amount of microscopic anatomy [9] [10] is revealed, [7] [5] [4] and can be used to diagnose a wide range of histopathologic conditions. [8]
Biological Stains (1925) History of Staining (1933; 1948) Staining Procedures (1944–55; 1960) Manual of Microbiological Methods (1957) Harold Joel Conn (May 29, 1886 – November 10, 1975) [ 1 ] was an American agricultural bacteriologist , known for his work on soil microbiology and bacterial staining techniques.
Immunohistochemistry can be performed on tissue that has been fixed and embedded in paraffin, but also cryopreservated (frozen) tissue.Based on the way the tissue is preserved, there are different steps to prepare the tissue for immunohistochemistry, but the general method includes proper fixation, antigen retrieval incubation with primary antibody, then incubation with secondary antibody.
Bismarck brown Y stains acid mucins to yellow color. It also stains mast cell granules brown. [3] It can be used with live cells.It is also used to stain cartilage in bone specimens, as one of Kasten's Schiff-type reagents in the periodic acid-Schiff stain to stain cellulose, and in Feulgen stain to stain DNA.