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  2. DNA sequencing - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencing

    DNA sequencing is the process of determining the nucleic acid sequence – the order of nucleotides in DNA. It includes any method or technology that is used to determine the order of the four bases: adenine, guanine, cytosine, and thymine. The advent of rapid DNA sequencing methods has greatly accelerated biological and medical research and ...

  3. Nucleic acid methods - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_methods

    Download as PDF; Printable version; ... Nucleic acid methods are the techniques used to study nucleic acids: ... DNA sequencing;

  4. DNA sequencer - Wikipedia

    en.wikipedia.org/wiki/DNA_sequencer

    The first DNA sequencing methods were developed by Gilbert (1973) [8] and Sanger (1975). [9] Gilbert introduced a sequencing method based on chemical modification of DNA followed by cleavage at specific bases whereas Sanger's technique is based on dideoxynucleotide chain termination. The Sanger method became popular due to its increased ...

  5. DNA microarray - Wikipedia

    en.wikipedia.org/wiki/DNA_microarray

    Probe-target hybridization is usually detected and quantified by detection of fluorophore-, silver-, or chemiluminescence-labeled targets to determine relative abundance of nucleic acid sequences in the target. The original nucleic acid arrays were macro arrays approximately 9 cm × 12 cm and the first computerized image based analysis was ...

  6. Nucleic acid sequence - Wikipedia

    en.wikipedia.org/wiki/Nucleic_acid_sequence

    The sequence of nucleobases on a nucleic acid strand is translated by cell machinery into a sequence of amino acids making up a protein strand. Each group of three bases, called a codon , corresponds to a single amino acid, and there is a specific genetic code by which each possible combination of three bases corresponds to a specific amino acid.

  7. Single-cell sequencing - Wikipedia

    en.wikipedia.org/wiki/Single-cell_sequencing

    Single-cell sequencing examines the nucleic acid sequence information from individual cells with optimized next-generation sequencing technologies, providing a higher resolution of cellular differences and a better understanding of the function of an individual cell in the context of its microenvironment. [1]

  8. SNP array - Wikipedia

    en.wikipedia.org/wiki/SNP_array

    Fragmented nucleic acid sequences of target, labelled with fluorescent dyes. A detection system that records and interprets the hybridization signal. The ASO probes are often chosen based on sequencing of a representative panel of individuals: positions found to vary in the panel at a specified frequency are used as the basis for probes.

  9. Single-molecule real-time sequencing - Wikipedia

    en.wikipedia.org/wiki/Single-molecule_real-time...

    The DNA sequencing is done on a chip that contains many ZMWs. Inside each ZMW, a single active DNA polymerase with a single molecule of single stranded DNA template is immobilized to the bottom through which light can penetrate and create a visualization chamber that allows monitoring of the activity of the DNA polymerase at a single molecule level.