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  2. Expression vector - Wikipedia

    en.wikipedia.org/wiki/Expression_vector

    A cell line derived from the cabbage looper is of particular interest, as it has been developed to grow fast and without the expensive serum normally needed to boost cell growth. [ 27 ] [ 28 ] The shuttle vector is called bacmid, and gene expression is under the control of a strong promoter pPolh. [ 29 ]

  3. Cre-Lox recombination - Wikipedia

    en.wikipedia.org/wiki/Cre-Lox_recombination

    Cre-Lox recombination is a special type of site-specific recombination developed by Dr. Brian Sauer and patented by DuPont that operated in both mitotic and non-mitotic cells, and was initially used in activating gene expression in mammalian cell lines. [3] [4] [5] Subsequently, researchers in the laboratory of Dr. Jamey Marth demonstrated that ...

  4. Sleeping Beauty transposon system - Wikipedia

    en.wikipedia.org/wiki/Sleeping_Beauty_transposon...

    Middle lines: Sleeping Beauty (SB) transposase binds to the IR/DRs as shown and cuts the transposon out of the plasmid (the cut sites are indicated by the two black slashed lines in the remaining plasmid) Bottom two lines: Another DNA molecule (green) with a TA sequence can become the recipient of a transposed transposon. In the process, the TA ...

  5. Gene therapy - Wikipedia

    en.wikipedia.org/wiki/Gene_therapy

    Retroviruses and stem cells were mixed, after which the viruses inserted the gene into the stem cell chromosomes. Stem cells containing the working ADA gene were injected into Andrew's blood. Injections of the ADA enzyme were also given weekly. For four years T cells (white blood cells), produced by stem cells, made ADA enzymes using the ADA ...

  6. Type 3c diabetes - Wikipedia

    en.wikipedia.org/wiki/Type_3c_diabetes

    Type 3c diabetes (also known as pancreatogenic diabetes) is diabetes that comes secondary to pancreatic diseases, [1] involving the exocrine and digestive functions of the pancreas. It also occurs following surgical removal of the pancreas. Around 5–10% of cases of diabetes in the Western world are related to pancreatic diseases.

  7. Epithelial sodium channel - Wikipedia

    en.wikipedia.org/wiki/Epithelial_sodium_channel

    The epithelial sodium channel (ENaC), (also known as amiloride-sensitive sodium channel) is a membrane-bound ion channel that is selectively permeable to sodium ions (Na +).It is assembled as a heterotrimer composed of three homologous subunits α or δ, β, and γ, [2] These subunits are encoded by four genes: SCNN1A, SCNN1B, SCNN1G, and SCNN1D.

  8. Diabetes management - Wikipedia

    en.wikipedia.org/wiki/Diabetes_management

    Control of diabetes may be improved by patients using home glucose meters to regularly measure their glucose levels. [8] An image of a continuous glucose monitor attached on the skin. Continuous glucose monitors are another method to measure BG levels. A CGM is a device that sits on the surface of the skin and measures the amount of glucose ...

  9. Indoleamine 2,3-dioxygenase - Wikipedia

    en.wikipedia.org/wiki/Indoleamine_2,3-dioxygenase

    Indoleamine 2,3-dioxygenase is the first and rate-limiting enzyme of tryptophan catabolism through the kynurenine pathway.. IDO is an important molecule in the mechanisms of tolerance and its physiological functions include the suppression of potentially dangerous inflammatory processes in the body. [16]

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