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False negative readings can occur when testing is done too early. hCG levels rise rapidly in early pregnancy and the chances of false negative test results diminish with time (increasing gestational age). [22] Less sensitive urine tests and qualitative blood tests may not detect pregnancy until three or four days after implantation. [23]
A later alternative to the rabbit test, known as the "Hogben test", used the African clawed frog, and yielded results without the need to cut the animal open. [6] Modern pregnancy tests continue to operate on the basis of testing for the presence of the hormone hCG in the blood or urine, but they no longer require the use of a live animal.
Blood and urine tests can detect pregnancy by 11 and 14 days, respectively, after fertilization. [78] [79] Blood pregnancy tests are more sensitive than urine tests (giving fewer false negatives). [80] Home pregnancy tests are urine tests, and normally detect a pregnancy 12 to 15 days after fertilization. [81]
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An example of an algorithm for determining the indication for prenatal genetic testing for trisomy 21 (Down syndrome), wherein the genetic blood test (in center) is performed by detecting cffDNA in a blood sample from the mother. [60] Trisomy 21. Fetal trisomy of chromosome 21 is the cause of Down's syndrome.
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Around weeks 11–13, nuchal translucency scan (NT) may be offered which can be combined with blood tests for PAPP-A and beta-hCG, two serum markers that correlate with chromosomal abnormalities, in what is called the First Trimester Combined Test. The results of the blood test are then combined with the NT ultrasound measurements, maternal age ...
Tests results can be available in as little as a few minutes. Generally there is a trade off between time and sensitivity: more sensitive tests may take longer to develop. The other key advantage of this format of test compared to other immunoassays is the simplicity of the test, by typically requiring little or no sample or reagent preparation ...
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