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Primer Premier is a bioinformatics software used for various PCR applications. It supports the design of degenerate primers for amplifying a related set of nucleotide sequences for the detection of common traits amongst organisms, as well as to determine heredity. [1] The software also designs tagged and nested primers for multiplex PCR ...
Beacon Designer designs primers and probes for real-time PCR (polymerase chain reaction) assays.It is compatible to work on Windows as well as on Mac. The software currently supports the following real-time PCR chemistries for primer and probe design:
OLIGO Primer Analysis Software is a software for DNA primer design. [ 1 ] [ 2 ] The first paper describing this software was published in 1989. [ 3 ] The program is a real time PCR primer and probe search and analysis tool.
UGENE is computer software for bioinformatics. [1] [2] It works on personal computer operating systems such as Windows, macOS, or Linux.It is released as free and open-source software, under a GNU General Public License (GPL) version 2.
NetPrimer is a gratis web-based tool used for analysing primers used in PCR to amplify a DNA sequence. [2] The software predicts the melting temperature of the primers using the nearest neighbor thermodynamic algorithm. The accurate prediction of the melting temperature (Tm) is one of the most important factors that governs the success of a PCR ...
The design of appropriate short or long primer pairs is only one goal of PCR product prediction. Other information provided by in silico PCR tools may include determining primer location, orientation, length of each amplicon, simulation of electrophoretic mobility, identification of open reading frames, and links to other web resources. [7] [8] [9]
I have very good experience with primer3 in picking primers working in experiments later. --Untergasser 14:33, 4 July 2006 (UTC) I also use primer3 to help me design primers. Can a link to primer3 be added in addition to the other free (Primerfox) primer designing program? --Lunajurai 20:06, 18 January 2008 (UTC)
PCR Primer design - easy primer design and testing. Also uses primer3; Agarose Gel simulation. CRISPR INDEL analysis. MacVector has a contig assembly plugin called Assembler that uses phred, phrap, Bowtie, SPAdes, Velvet and cross match. As of version 13.0.1 MacVector uses Sparkle for updating between releases.