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Xylose lysine deoxycholate agar (XLD agar) is a selective growth medium used in the isolation of Salmonella and Shigella species from clinical samples and from food. [ 1 ] [ 2 ] The agar was developed by Welton Taylor in 1965. [ 3 ]
XLT Agar (Xylose Lysine Tergitol-4) is a selective culture medium for the isolation and identification of salmonellae from food and environmental samples. It is similar to XLD agar; however, the agar is supplemented with the surfactant, Tergitol 4, which causes inhibition of Proteus spp. and other non-Salmonellae. [1]
XLD may refer to: Xylose lysine deoxycholate or XLD agar, a growth medium for bacterial cultures; The XLD connector, a keyed variant of the XLR connector;
As there are many bacteria that also look like Salmonella on DCA, it is widely recommended that more selective agars are used for the identification of Salmonella, namely xylose lysine deoxycholate (XLD) agar. This growth medium is heat-sensitive and should be poured and cooled as soon as possible after addition of the deoxycholate, otherwise ...
pH adjusted to neutral (6.8) at 25 °C (77 °F). Nutrient broth has the same composition, but lacks agar. [2] [3] These ingredients are combined and boiled for approximately one minute to ensure they are mixed and then sterilized by autoclaving, typically at 121 °C (250 °F) for 15 minutes.
After 24 hours of growth, this image depicts four different agar media culture plates that had been inoculated with Shigella sp., Escherichia sp., and Proteus sp. bacteria, (clockwise: MacConkey, Shigella-Salmonella, Bismuth Sulfite, and Brilliant Green agars).
Proper autoclave treatment will inactivate all resistant bacterial spores in addition to fungi, bacteria, and viruses, but is not expected to eliminate all prions, which vary in their heat resistance. For prion elimination, various recommendations state 121–132 °C (250–270 °F) for 60 minutes or 134 °C (273 °F) for at least 18 minutes. [14]
Welton Ivan Taylor (November 12, 1919 – November 1, 2012) was an American microbiologist, inventor and civil rights activist.He is known for his work on food-borne pathogens, notably for developing tests for Salmonella and for inventing the XLD (Xylose Lysine Deoxycholate) agar, which can be used to isolate Salmonella and Shigella bacteria.