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  2. Genetic engineering techniques - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering_techniques

    Genetic engineering techniques allow the modification of animal and plant genomes. Techniques have been devised to insert, delete, and modify DNA at multiple levels, ranging from a specific base pair in a specific gene to entire genes. There are a number of steps that are followed before a genetically modified organism (GMO) is created.

  3. Genetic engineering - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering

    This is an accepted version of this page This is the latest accepted revision, reviewed on 4 February 2025. Manipulation of an organism's genome For a non-technical introduction to the topic of genetics, see Introduction to genetics. For the song by Orchestral Manoeuvres in the Dark, see Genetic Engineering (song). For the Montreal hardcore band, see Genetic Control. Part of a series on ...

  4. Gene targeting - Wikipedia

    en.wikipedia.org/wiki/Gene_targeting

    The two most established forms of gene editing are gene-targeting and targeted-mutagenesis. While gene targeting relies on the Homology Directed Repair (HDR) (also called Homologous Recombination, HR) DNA repair pathway, targeted-mutagenesis uses Non-Homologous-End-Joining (NHEJ) of broken DNA. NHEJ is an error-prone DNA repair pathway, meaning ...

  5. Genetically modified crops - Wikipedia

    en.wikipedia.org/wiki/Genetically_modified_crops

    Over-reliance on glyphosate and a reduction in the diversity of weed management practices allowed the spread of glyphosate resistance in 14 weed species in the US, [207] and in soybeans. [ 5 ] To reduce resistance to Bacillus thuringiensis (Bt) crops, the 1996 commercialization of transgenic cotton and maize came with a management strategy to ...

  6. Genetically modified soybean - Wikipedia

    en.wikipedia.org/wiki/Genetically_modified_soybean

    Monsanto developed a glyphosate-resistant soybean that also expresses Cry1Ac protein from Bacillus thuringiensis and the glyphosate-resistance gene, which completed the Brazilian regulatory process in 2010. This is a cross of two events, MON87701 x MON89788.

  7. CRISPR gene editing - Wikipedia

    en.wikipedia.org/wiki/CRISPR_gene_editing

    CRISPR-Cas9 genome editing techniques have many potential applications. The use of the CRISPR-Cas9-gRNA complex for genome editing [10] was the AAAS's choice for Breakthrough of the Year in 2015. [11] Many bioethical concerns have been raised about the prospect of using CRISPR for germline editing, especially in human embryos. [12]

  8. Genetically modified food - Wikipedia

    en.wikipedia.org/wiki/Genetically_modified_food

    Creating genetically modified food is a multi-step process. The first step is to identify a useful gene from another organism that you would like to add. The gene can be taken from a cell [72] or artificially synthesised, [73] and then combined with other genetic elements, including a promoter and terminator region and a selectable marker. [74]

  9. Genetically modified food controversies - Wikipedia

    en.wikipedia.org/wiki/Genetically_modified_food...

    Bushel per acre yield changes were +6.4 for European corn borer resistance, +5.76 for glufosinate tolerance, −5.98 for glyphosate tolerance and −12.22 for corn rootworm resistance. The study found interactions among the genes in multi-trait hybrid strains, such that the net effect varied from the sum of the individual effects.