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Chemiluminescence differs from fluorescence or phosphorescence in that the electronic excited state is the product of a chemical reaction rather than of the absorption of a photon. It is the antithesis of a photochemical reaction, in which light is used to drive an endothermic chemical reaction.
Electrochemiluminescence or electrogenerated chemiluminescence (ECL) is a kind of luminescence produced during electrochemical reactions in solutions. In electrogenerated chemiluminescence, electrochemically generated intermediates undergo a highly exergonic reaction to produce an electronically excited state that then emits light upon relaxation to a lower-level state.
Luminol (C 8 H 7 N 3 O 2) is a chemical that exhibits chemiluminescence, with a blue glow, when mixed with an appropriate oxidizing agent.Luminol is a white-to-pale-yellow crystalline solid that is soluble in most polar organic solvents but insoluble in water.
In chemiluminescence, an excited state is created via a chemical reaction. The light emission tracks the kinetic progress of the underlying chemical reaction. The excited state will then transfer to a dye molecule, also known as a sensitizer or fluorophor, and subsequently fluoresce back to the ground state.
During chemiluminescence, the vibrationally excited product of an exoergic chemical reaction relaxes to its ground state with the emission of photons. [1] Since the process does not require excitation light, problems in its application caused by light scattering or source instability are absent, and there is no concern about autofluorescence in ...
Chemiluminescence, the emission of light as a result of a chemical reaction. Bioluminescence, a result of biochemical reactions in a living organism; Electrochemiluminescence, a result of an electrochemical reaction; Lyoluminescence, a result of dissolving a solid (usually heavily irradiated) in a liquid solvent
Luminescence is the result of a chemical or biochemical reaction. Luminescence detection is simpler optically than fluorescence detection because luminescence does not require a light source for excitation or optics for selecting discrete excitation wavelengths.
In other projects Wikidata item; Appearance. ... Chemiluminescent immunoassay (CLIA) is a type of immunoassay employing chemiluminescence. [1] [2] See also