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Gene length: Longer genes will have more fragments/reads/counts than shorter genes if transcript expression is the same. This is adjusted by dividing the FPM by the length of a feature (which can be a gene, transcript, or exon), resulting in the metric fragments per kilobase of feature per million mapped reads (FPKM). [90]
In the simplest cases, normalization of ratings means adjusting values measured on different scales to a notionally common scale, often prior to averaging. In more complicated cases, normalization may refer to more sophisticated adjustments where the intention is to bring the entire probability distributions of adjusted values into alignment.
In statistics, quantile normalization is a technique for making two distributions identical in statistical properties. To quantile-normalize a test distribution to a reference distribution of the same length, sort the test distribution and sort the reference distribution.
It is already adapted to align long reads (third-generation sequencing technologies) and can reach speeds of 45 million paired reads per hour per processor. [49] Subjunc [44] is a specialized version of Subread. It uses all mappable regions in an RNA-seq read to discover exons and exon-exon junctions.
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Before you have a potato emergency, read up on how to thicken mashed potatoes. You can turn a watery mash into fluffy perfection with these easy methods.
While Thornton’s performance, and Sheridan’s writing of his character, have been praised (Thornton recently received a Golden Globe nomination for his work on the show), the actresses who play ...
Disintegrations per minute (dpm) and disintegrations per second (dps) are measures of the activity of the source of radioactivity. The SI unit of radioactivity, the becquerel (Bq), is equivalent to one disintegration per second. This unit should not be confused with cps, which is the number of counts received by an instrument from the source.