Search results
Results from the WOW.Com Content Network
Laccase is produced by a number of fungal species that can infect grapes, most notably Botrytis cinerea Pers. (1794). [17] Laccase is active at wine pH and its activity is not readily suppressed by sulfur dioxide. It has been noted to cause oxidative browning in white wines and loss of colour in red wines.
Laccase EC 1.10.3.2 (urishiol oxidase), a 3-domain enzyme found in fungi and plants, which oxidizes different phenols and diamines. CueO is a laccase found in Escherichia coli that is involved in copper-resistance. [4] Ascorbate oxidase EC 1.10.3.3, a 3-domain enzyme found in higher plants.
Oxidative enzymes such as phenol oxidase and peroxidase mediate lignin degradation and humification. [47] Phenol oxidase activity is quantified by oxidation of L-3, 4-dihydoxyphenylalanine (L-DOPA), pyrogallol (1, 2, 3-trihydroxybenzene), or ABTS (2, 2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid).
[41] [42] Though the metabolic degradation pathways are not fully understood, enzymes including phenoloxidase laccase (EC 1.10.3.2) and laccase-like enzymes are involved in the oxidation of aromatic substrates. [40] [43] [44] These exoenzymes can be potentially applied in the environmental degradation of phenolic pollutants.
Water is released, and the second o-quinone product is formed together with the restoration of the initial Cu(II)-Cu(II) state to complete the catalytic cycle. [ 15 ] This proposed catalytic cycle is supported by the experimental observation that stoichiometric amounts of o-quinone form after catechol addition to the enzyme, even when dioxygen ...
Phenol is an organic compound appreciably soluble in water, with about 84.2 g dissolving in 1000 ml (0.895 M). Homogeneous mixtures of phenol and water at phenol to water mass ratios of ~2.6 and higher are possible. The sodium salt of phenol, sodium phenoxide, is far more water-soluble. It is a combustible solid (NFPA rating = 2).
In female elephants, the two compounds 3-ethyl phenol and 2-ethyl 4,5 dimethylphenol have been detected in urine samples. [70] Temporal glands secretion examination showed the presence of phenol, m-cresol and p-cresol (4-methyl phenol) during musth in male elephants. [71] [72] [73] p-Cresol and o-cresol are also components of the human sweat.
Phenol: The phenol used for biochemistry comes as a water-saturated solution with Tris buffer, as a Tris-buffered 50% phenol, 50% chloroform solution, or as a Tris-buffered 50% phenol, 48% chloroform, 2% isoamyl alcohol solution (sometimes called "25:24:1"). Phenol is naturally somewhat water-soluble, and gives a fuzzy interface, which is ...