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  2. High-performance liquid chromatography - Wikipedia

    en.wikipedia.org/wiki/High-performance_liquid...

    HPLC is distinguished from traditional ("low pressure") liquid chromatography because operational pressures are significantly higher (around 50–1400 bar), while ordinary liquid chromatography typically relies on the force of gravity to pass the mobile phase through the packed column. Due to the small sample amount separated in analytical HPLC ...

  3. Chromatography - Wikipedia

    en.wikipedia.org/wiki/Chromatography

    Liquid chromatography (LC) is a separation technique in which the mobile phase is a liquid. It can be carried out either in a column or a plane. Present day liquid chromatography that generally utilizes very small packing particles and a relatively high pressure is referred to as high-performance liquid chromatography.

  4. Capillary electrochromatography - Wikipedia

    en.wikipedia.org/.../Capillary_electrochromatography

    Capillary electrophoresis aims to separate analytes on the basis of their mass-to-charge ratio by passing a high voltage across ends of a capillary tube, which is filled with the analyte. High-performance liquid chromatography separates analytes by passing them, under high pressure, through a column filled with stationary phase. The ...

  5. Atmospheric-pressure chemical ionization - Wikipedia

    en.wikipedia.org/wiki/Atmospheric-pressure...

    Atmospheric pressure chemical ionization chamber cross section. Atmospheric pressure chemical ionization (APCI) is an ionization method used in mass spectrometry which utilizes gas-phase ion-molecule reactions at atmospheric pressure (10 5 Pa), [1] [2] commonly coupled with high-performance liquid chromatography (HPLC). [3]

  6. Monolithic HPLC column - Wikipedia

    en.wikipedia.org/wiki/Monolithic_HPLC_column

    In order to withstand the pressure, a new field of chromatography came into being: UHPLC or UPLC- ultra high pressure liquid chromatography. The new instruments were able to endure pressures of up to 15,000 pounds per square inch (1,000 bar), as opposed to conventional machines, which, as previously state, can hold up to 5,000 pounds per square ...

  7. Molar mass distribution - Wikipedia

    en.wikipedia.org/wiki/Molar_mass_distribution

    The most common technique for measuring molecular mass used in modern times is a variant of high-pressure liquid chromatography (HPLC) known by the interchangeable terms of size exclusion chromatography (SEC) and gel permeation chromatography (GPC).

  8. Column chromatography - Wikipedia

    en.wikipedia.org/wiki/Column_chromatography

    However, the use of this small packing media causes the high back pressure and is why it is termed high pressure liquid chromatography. The LPLC columns are typically packed with silica of around 50 micrometres, thus reducing back pressure and resolution, but it also removes the need for expensive high pressure pumps.

  9. Countercurrent chromatography - Wikipedia

    en.wikipedia.org/wiki/Countercurrent_chromatography

    A high-performance countercurrent chromatography system. Countercurrent chromatography (CCC, also counter-current chromatography) is a form of liquidliquid chromatography that uses a liquid stationary phase that is held in place by inertia of the molecules composing the stationary phase accelerating toward the center of a centrifuge due to centripetal force [1] and is used to separate ...

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