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  2. Eukaryotic DNA replication - Wikipedia

    en.wikipedia.org/wiki/Eukaryotic_DNA_replication

    After around 20 nucleotides, elongation is taken over by Pol ε on the leading strand and Pol δ on the lagging strand. [103] Polymerase δ (Pol δ): Highly processive and has proofreading, 3'->5' exonuclease activity. In vivo, it is the main polymerase involved in both lagging strand and leading strand synthesis. [104]

  3. DNA replication - Wikipedia

    en.wikipedia.org/wiki/DNA_replication

    DNA is read by DNA polymerase in the 3′ to 5′ direction, meaning the new strand is synthesized in the 5' to 3' direction. Since the leading and lagging strand templates are oriented in opposite directions at the replication fork, a major issue is how to achieve synthesis of new lagging strand DNA, whose direction of synthesis is opposite to ...

  4. DNA polymerase I - Wikipedia

    en.wikipedia.org/wiki/DNA_polymerase_I

    In DNA replication, the leading DNA strand is continuously extended in the direction of replication fork movement, whereas the DNA lagging strand runs discontinuously in the opposite direction as Okazaki fragments. [7] DNA polymerases also cannot initiate DNA chains so they must be initiated by short RNA or DNA segments known as primers. [5]

  5. Primer binding site - Wikipedia

    en.wikipedia.org/wiki/Primer_binding_site

    The lagging strand moves away from the replication fork in the 3' to 5' direction and consists of small fragments called Okazaki fragments. DNA polymerase makes the lagging strand by using a new RNA primer for each Okazaki fragment it encounters. Overall, the leading strand only uses one RNA primer, while the lagging strand uses a new RNA ...

  6. Helicase - Wikipedia

    en.wikipedia.org/wiki/Helicase

    Helicase polarity, which is also deemed "directionality", is defined as the direction (characterized as 5'→3' or 3'→5') of helicase movement on the DNA/RNA single-strand along which it is moving. This determination of polarity is vital in f.ex. determining whether the tested helicase attaches to the DNA leading strand, or the DNA lagging ...

  7. Primer (molecular biology) - Wikipedia

    en.wikipedia.org/wiki/Primer_(molecular_biology)

    In eukaryotes the removal of RNA primers in the lagging strand is essential for the completion of replication. Thus, as the lagging strand being synthesized by DNA polymerase δ in 5′→3′ direction, Okazaki fragments are formed, which are discontinuous strands of DNA. Then, when the DNA polymerase reaches to the 5’ end of the RNA primer ...

  8. DNA polymerase delta - Wikipedia

    en.wikipedia.org/wiki/DNA_polymerase_delta

    DNA Pol δ is an enzyme used for both leading and lagging strand synthesis. [ 2 ] [ 3 ] It exhibits increased processivity when interacting with the proliferating cell nuclear antigen ( PCNA ). As well, the multisubunit protein replication factor C , through its role as the clamp loader for PCNA (which involves catalysing the loading of PCNA on ...

  9. GC skew - Wikipedia

    en.wikipedia.org/wiki/GC_skew

    This asymmetry is due to the formation of the replication fork and its division into nascent leading and lagging strands. The leading strand is synthesized continuously and in juxtapose to the leading strand; the lagging strand is replicated through short fragments of polynucleotide (Okazaki fragments) in a 5' to 3' direction. [6]