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The Henderson–Hasselbalch equation can be used to model these equilibria. It is important to maintain this pH of 7.4 to ensure enzymes are able to work optimally. [10] Life threatening Acidosis (a low blood pH resulting in nausea, headaches, and even coma, and convulsions) is due to a lack of functioning of enzymes at a low pH. [10]
A strong acid, such as hydrochloric acid, at concentration 1 mol dm −3 has a pH of 0, while a strong alkali like sodium hydroxide, at the same concentration, has a pH of 14. Since pH is a logarithmic scale, a difference of one in pH is equivalent to a tenfold difference in hydrogen ion concentration.
For example, if the concentration of the conjugate base is 10 times greater than the concentration of the acid, their ratio is 10:1, and consequently the pH is pK a + 1 or pK b + 1. Conversely, if a 10-fold excess of the acid occurs with respect to the base, the ratio is 1:10 and the pH is pK a − 1 or pK b − 1.
Logarithmic dilution. A serial dilution is the step-wise dilution of a substance in solution, either by using a constant dilution factor, or by using a variable factor between dilutions. If the dilution factor at each step is constant, this results in a geometric progression of the concentration in a logarithmic fashion.
The logarithmic decrement can be obtained e.g. as ln(x 1 /x 3).Logarithmic decrement, , is used to find the damping ratio of an underdamped system in the time domain.. The method of logarithmic decrement becomes less and less precise as the damping ratio increases past about 0.5; it does not apply at all for a damping ratio greater than 1.0 because the system is overdamped.
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For general purposes, buffers at pH 4.00 and pH 10.00 are suitable. The pH meter has one calibration control to set the meter reading equal to the value of the first standard buffer and a second control to adjust the meter reading to the value of the second buffer. A third control allows the temperature to be set.
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