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Immunodiffusion principles and application. Archived from the original on 2019-12-11 Photographs of Ouchterlony immunodiffusion patterns showing stained precipitin lines of full identity, partial identity and non-identity. "Ouchterlony Assay". YouTube. Bio-Rad Laboratories. October 16, 2012.
Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium. [1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar , resulting in the formation of a visible precipitate when they interact.
Örjan Thomas Ouchterlony (January 14, 1914, Stockholm – September 25, 2004) was a Swedish bacteriologist and immunologist who is credited with the creation of the Ouchterlony double immuno diffusion test in the 1940s. [1] [2] He was trained at Karolinska Institute, where his received his medical doctorate.
Immunocytochemistry is a technique used to assess the presence of a specific protein or antigen in cells (cultured cells, cell suspensions) by use of a specific antibody, which binds to it, thereby allowing visualization and examination under a microscope. It is a valuable tool for the determination of cellular contents from individual cells.
Ouchterlony double immunodiffusion, an immunological technique named after Örjan Ouchterlony Topics referred to by the same term This disambiguation page lists articles associated with the title Ochterlony .
Introductory video demonstrating radial immunodiffusion technique (3:43 minutes). "Radial Immunodiffusion (Mancini Technique)" (video). Frank Lectures. 2017-08-08. Archived from the original on 2021-12-21 – via YouTube. Introductory lecture/slideshow illustrating radial immunodiffusion theory and technique. (6:56 minutes)
A radioimmunoassay (RIA) is an immunoassay that uses radiolabeled molecules in a stepwise formation of immune complexes.A RIA is a very sensitive in vitro assay technique used to measure concentrations of substances, usually measuring antigen concentrations (for example, hormone levels in blood) by use of antibodies.
Immunoelectrophoresis is a general term describing many combinations of the principles of electrophoresis and reaction of antibodies, also known as immunodiffusion. [1] Agarose as 1% gel slabs of about 1 mm thickness buffered at high pH (around 8.6) is traditionally preferred for electrophoresis and the reaction with antibodies. The agarose was ...