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Benedict's reagent (often called Benedict's qualitative solution or Benedict's solution) is a chemical reagent and complex mixture of sodium carbonate, sodium citrate, and copper(II) sulfate pentahydrate. [1] It is often used in place of Fehling's solution to detect the presence of reducing sugars and other reducing substances. [2]
Reducing form of glucose (the aldehyde group is on the far right). A reducing sugar is any sugar that is capable of acting as a reducing agent. [1] In an alkaline solution, a reducing sugar forms some aldehyde or ketone, which allows it to act as a reducing agent, for example in Benedict's reagent.
It is advised to check the references for photos of reaction results. [1] Reagent testers might show the colour of the desired substance while not showing a different colour for a more dangerous additive. [2]
[citation needed] The pH range of a solution of 5 g/100 ml water at 25 °C is 7.5 to 9.0. It is added to many commercially packaged dairy products to control the pH impact of the gastrointestinal system of humans [ citation needed ] , mainly in processed products such as cheese and yogurt, although it also has beneficial effects on the physical ...
Citric acid is used as an odorless alternative to white vinegar for fabric dyeing with acid dyes. Sodium citrate is a component of Benedict's reagent, used for both qualitative and quantitative identification of reducing sugars. [39] Citric acid can be used as an alternative to nitric acid in passivation of stainless steel. [40]
Sucrose is a non-reducing sugar, so will not test positive with Benedict's solution. To test for sucrose, the sample is treated with sucrase. The sucrose is hydrolysed into glucose and fructose, with glucose being a reducing sugar, which in turn tests positive with Benedict's solution. [citation needed].
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When added to a solution containing ketoses, a red color is formed rapidly indicating a positive test. When added to a solution containing aldoses, a slower forming light pink is observed instead. Seliwanoff-Reaction. The reagents consist of resorcinol and concentrated hydrochloric acid: