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Cytology is the name given to the branch of biology that deals with the formation, structure and functionality of the cells. [1] Liver cytology specializes in the study of liver cells. The main liver cells are called hepatocytes; however, there are other cells that can be observed in a liver sample such as Kupffer cells (macrophages). [2]
For these purposes, hepatocytes are usually isolated from animal or human [8] whole liver or liver tissue by collagenase digestion, which is a two-step process. In the first step, the liver is placed in an isotonic solution, in which calcium is removed to disrupt cell-cell tight junctions by the use of a calcium chelating agent .
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The Terminologia Histologica (TH) is the controlled vocabulary for use in cytology and histology. [1] [2] In April 2011, Terminologia Histologica was published online [3] by the Federative International Programme on Anatomical Terminologies (FIPAT), the successor of FCAT. It was intended to replace Nomina Histologica.
In histology (microscopic anatomy), the lobules of liver, or hepatic lobules, are small divisions of the liver defined at the microscopic scale. The hepatic lobule is a building block of the liver tissue , consisting of portal triads, hepatocytes arranged in linear cords between a capillary network, and a central vein .
Microscopic anatomy is the study of minute anatomical structures assisted with microscopes, and includes histology (the study of the organization of tissues), and cytology (the study of cells). The Anatomy Lesson of Dr. Nicolaes Tulp , by Rembrandt ; depicts an anatomy demonstration using a cadaver .
Articles reating to the liver, a major organ, only found in vertebrates, which performs many essential biological functions such as detoxification of the organism, and the synthesis of proteins and biochemicals necessary for digestion and growth.
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