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The refractive indices of the oil and of the glass in the first lens element are nearly the same, which means that the refraction of light will be small upon entering the lens (the oil and glass are optically very similar). The correct immersion oil for an objective lens has to be used to ensure that the refractive indices match closely. Use of ...
Inverted microscopes are useful for observing living cells or organisms at the bottom of a large container (e.g., a tissue culture flask) under more natural conditions than on a glass slide, as is the case with a conventional microscope. An inverted microscope is also used for visualisation of the Mycobacterium tuberculosis bacteria in the ...
Leica 60 mm Macro-Elmarit-R 1st version – 1972 – outside bayonet lens hood fitting; Leica 60 mm Macro-Elmarit-R dn2 version; Leica 75 mm f /2.0 Elcan-R code C-341 – extremely rare; Leica 80mm f /1.4 Summilux-R; Elmarit-R 135 mm. Leica 90 mm f /2.8 Elmarit-R 1st version – 1964–1996; Leica 90 mm f /2.8 Elmarit-R 2nd version – 1983
As with objective lenses, a condenser lens with a maximum numerical aperture of greater than 0.95 is designed to be used under oil immersion (or, more rarely, under water immersion), with a layer of immersion oil placed in contact with both the slide/coverslip and the lens of the condenser. An oil immersion condenser may typically have NA of up ...
Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...
Leeuwenhoek and Kircher used a "simple microscope" for their work. A special form of the simple microscope was the compass microscope, which was built from the end of the 17th century. Like a pair of compasses, it had two legs, the distance between which could be adjusted in fine steps. On one leg was the objective lens, and the specimen was ...
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