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Nile red (also known as Nile blue oxazone) is a lipophilic stain. Nile red stains intracellular lipid droplets yellow. In most polar solvents, Nile red will not fluoresce; however, when in a lipid-rich environment, it can be intensely fluorescent, with varying colors from deep red (for polar membrane lipid) to strong yellow-gold emission (for neutral lipid in intracellular storages).
Nile red (also known as Nile blue oxazone) is formed by boiling Nile blue with sulfuric acid. This produces a mix of Nile red and Nile blue. Nile red is a lipophilic stain; it will accumulate in lipid globules inside cells, staining them red. Nile red can be used with living cells.
Plants are important for the environment and ecosystems so the plastics are damaging to plants and organisms living in these ecosystems. [102] Microplastics alter soil biophysical properties which affect the quality of the soil. This affects soil biological activity, biodiversity and plant health. Microplastics in the soil alter a plant's growth.
Microplastics are likely to degrade into smaller nanoplastics through chemical weathering processes, mechanical breakdown, and even through the digestive processes of animals. Nanoplastics, or NPs, are a subset of microplastics and they are smaller than 1 μm (1 micrometer or 1000 nm).
Plastics accounts for 80% of waste dispersed in the marine and coastal environment of the Mediterranean Sea. [24] Recent studies focus on the types of plastics found and primarily on the issue of microplastics, both at a global but also at a regional level, as in the case of the Mediterranean Sea, which was identified as a "target hotspot of the world" due to its amounts of microplastics ...
Microplastics are tiny pieces of plastic that are no larger than five millimeters (0.2 inches)—about the size of a single sesame seed—and form when larger plastics break down, either by ...
With respect to both plant microtechnique and animal microtechnique, four types of methods are commonly used, which are whole mounts, smears, squashes, and sections, in recent micro experiments. [2] Plant microtechnique contains direct macroscopic examinations, freehand sections, clearing, maceration, embedding, and staining. [ 3 ]
Ponceau 2R, Xylidine ponceau, Ponceau G, Red R, Acid Red 26, Food Red 5, or C.I. 16150 is a red azo dye used in histology for staining. It is easily soluble in water and slightly in ethanol . It usually comes as a disodium salt.