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The term "vital stain" is used by some authors to refer specifically to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell being examined is still alive. As the cells are alive and unfixed, outside the body, supravital stains are temporary in nature. [1] [2]
Heinz body stain of feline blood, showing three distinct Heinz bodies. Heinz bodies appear as small round inclusions within the red cell body, though they are not visible when stained with Romanowsky dyes. They are visualized more clearly with supravital staining [5] [6] (e.g., with new methylene blue, crystal violet or bromocresol green).
In supravital staining, living cells have been removed from an organism, whereas intravital staining is done by injecting or otherwise introducing the stain into the body. The term vital stain is used by some authors to refer to an intravital stain, and by others interchangeably with a supravital stain, the core concept being that the cell ...
For example, tissues that stain with an azure stain may be referred to as azurophilic. This may also be used for more generalized staining properties, such as acidophilic for tissues that stain by acidic stains (most notably eosin ), basophilic when staining in basic dyes, and amphophilic [ 25 ] when staining with either acid or basic dyes.
Pappenheimer bodies (Peripheral Blood / May-Grünwald Giemsa and Prussian blue stain) Pappenheimer bodies are abnormal basophilic granules of iron found inside red blood cells on routine blood stain. [1] They are a type of inclusion body composed of ferritin aggregates, or mitochondria or phagosomes containing aggregated ferritin. They appear ...
Pages in category "Body fluids" The following 53 pages are in this category, out of 53 total. This list may not reflect recent changes. ...
Testing for different body fluids with traditional serological techniques, such as those listed above, is possible, but not without some drawbacks. Firstly, not all body fluids have a reliable confirmatory test, and those that do typically require a larger amount of the suspected stain in order to perform the confirmatory test.
It is a supravital stain. [2] It is closely related to methylene blue, an older stain in wide use. Safety. New methylene blue is toxic. Skin contact or inhalation ...