Ad
related to: monolayer cell culture
Search results
Results from the WOW.Com Content Network
Most cells require a surface or an artificial substrate to form an adherent culture as a monolayer (one single-cell thick), whereas others can be grown free floating in a medium as a suspension culture. [2] This is typically facilitated via use of a liquid, semi-solid, or solid growth medium, such as broth or agar.
The monolayer formation time or monolayer time is the length of time required, on average, for a surface to be covered by an adsorbate, such as oxygen sticking to fresh aluminum. If the adsorbate has a unity sticking coefficient , so that every molecule which reaches the surface sticks to it without re-evaporating, then the monolayer time is ...
Monolayer cultures refer to cell cultures where cells are grown in a single, flat layer on the surface of a culture dish or substrate. In a monolayer culture, cells adhere to the substrate and spread out in a two-dimensional arrangement. This type of cell culture is commonly used in laboratory settings for various purposes, including research ...
Adherent cell cultures are a type of cell culture that requires cells to be attached to a surface in order for growth to occur. [1] Most vertebrate derived cells (with the exception of hematopoietic cells) can be cultured and require a 2 dimensional monolayer that to facilitate cell adhesion and spreading. [2]
A cell line can be defined as a permanently established cell culture which will propagate forever. Investigators mostly get cell lines from other investigators or from cell banks (such as the American Type Culture Collection) , because its much easier than creating new one. In special cases, investigators are obligated to establish a cell line.
In cell culture biology, confluence refers to the percentage of the surface of a culture dish that is covered by adherent cells.For example, 50 percent confluence means roughly half of the surface is covered, while 100 percent confluence means the surface is completely covered by the cells, and no more room is left for the cells to grow as a monolayer. [1]
Despite such heterogeneity, Caco-2 cells are used in cell invasion studies, viral transfection research, and lipid transport. [7] Caco-2 cells may be used as a confluent monolayer on a cell culture insert filter (e.g., Transwell). In this format, Caco-2 cells form a polarized epithelial cell monolayer that provides a physical and biochemical ...
3T3-L1 cells, similar to their other 3T3 counterparts, are typically propagated as an adherent monolayer within a culture vessel. Basal cell culture medias for 3T3-L1 cells tends to contain a version of Dubelcco's Modified Eagle's Medium (), fetal bovine serum (FBS), and an antibiotic at specific levels dependent upon protocol. [9]
Ad
related to: monolayer cell culture