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This occurs in a region of the gel that has larger pores so that the gel matrix does not retard the migration during the focusing or "stacking" event. [10] [11] Separation of the proteins by size is achieved in the lower, "resolving" region of the gel. The resolving gel typically has a much smaller pore size, which leads to a sieving effect ...
Considering more male or more female births as equally likely, the probability of the observed outcome is 0.5 82, or about 1 in 4,836,000,000,000,000,000,000,000; in modern terms, this is the p-value. Arbuthnot concluded that this is too small to be due to chance and must instead be due to divine providence: "From whence it follows, that it is ...
Agarose gel has large pore size and good gel strength, making it suitable as an anticonvection medium for the electrophoresis of DNA and large protein molecules. The pore size of a 1% gel has been estimated from 100 nm to 200–500 nm, [4] [5] and its gel strength allows gels as dilute as 0.15% to form a slab for gel electrophoresis. [6]
This aerogel has remarkable thermal insulative properties, having an extremely low thermal conductivity: from 0.003 W·m −1 ·K −1 [64] in atmospheric pressure down to 0.004 W·m −1 ·K −1 [59] in modest vacuum, which correspond to R-values of 14 to 105 (US customary) or 3.0 to 22.2 (metric) for 3.5 in (89 mm) thickness. For comparison ...
For instance, to avoid having the sample size be too small to reject a null hypothesis, it is recommended that one specify a sufficient sample size from the beginning. It is advisable to define a small, medium and large effect size for each of a number of important statistical tests which are used to test the hypotheses. [24]
In scientific research, the null hypothesis (often denoted H 0) [1] is the claim that the effect being studied does not exist. [note 1] The null hypothesis can also be described as the hypothesis in which no relationship exists between two sets of data or variables being analyzed. If the null hypothesis is true, any experimentally observed ...
For a standard agarose gel electrophoresis, 0.7% gel concentration gives good separation or resolution of large 5–10kb DNA fragments, while 2% gel concentration gives good resolution for small 0.2–1kb fragments. Up to 3% gel concentration can be used for separating very tiny fragments but a vertical polyacrylamide gel would be more ...
In contrast, the addition of cholesterol to gel phase bilayers disrupts local packing order, increasing the diffusion coefficient [10] and decreasing the elastic modulus. Interactions of cholesterol with multi-component systems are even more complicated, as these can result in intricate phase diagrams . [ 11 ]