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Protein–lipid interaction is the influence of membrane proteins on the lipid physical state or vice versa.. The questions which are relevant to understanding of the structure and function of the membrane are: 1) Do intrinsic membrane proteins bind tightly to lipids (see annular lipid shell), and what is the nature of the layer of lipids adjacent to the protein?
Whereas the concept of water activity is widely known and utilized in the applied biosciences, its complement—the protein activity which quantitates protein–protein interactions—is much less familiar to bioscientists as it is more difficult to determine in dilute solutions of proteins; protein activity is also much harder to determine for ...
The lipid-anchored protein can be located on either side of the cell membrane. Thus, the lipid serves to anchor the protein to the cell membrane. [1] [2] They are a type of proteolipids. The lipid groups play a role in protein interaction and can contribute to the function of the protein to which it is attached. [2]
Protein–protein interaction prediction is a field combining bioinformatics and structural biology in an attempt to identify and catalog physical interactions between pairs or groups of proteins. Understanding protein–protein interactions is important for the investigation of intracellular signaling pathways, modelling of protein complex ...
Tandem affinity purification (TAP) is an immunoprecipitation-based purification technique for studying protein–protein interactions.The goal is to extract from a cell only the protein of interest, in complex with any other proteins it interacted with.
Phosphatidylinositol 4,5-bisphosphate or PtdIns(4,5)P 2, also known simply as PIP 2 or PI(4,5)P 2, is a minor phospholipid component of cell membranes. PtdIns(4,5)P 2 is enriched at the plasma membrane where it is a substrate for a number of important signaling proteins. [1]
A protein microarray (or protein chip) is a high-throughput method used to track the interactions and activities of proteins, and to determine their function, and determining function on a large scale. [1] Its main advantage lies in the fact that large numbers of proteins can be tracked in parallel.
Pages in category "Protein–protein interaction assays" The following 26 pages are in this category, out of 26 total. This list may not reflect recent changes .