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"Ouchterlony double immunodiffusion" (photograph) permanent dead link ] Photograph of Ouchterlony double immunodiffusion plate with unstained precipitin lines of full identity and non-identity. "Diffusion Patterns". Immunodiffusion principles and application. Archived from the original on 2019-12-11
Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium. [1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar, resulting in the formation of a visible precipitate when they interact.
The six main antigens used in immunological laboratories for detection are Ro, La, Sm, RNP, Scl-70 and Jo1, [7] which are screened for by Ouchterlony double immuno diffusion techniques and confirmed by immunoblotting. On anti-nuclear antibody tests, these antigens have a speckled pattern. [8]
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Surface plasmon resonance is an example of technique that can detect binding between an unlabeled antibody and antigens. [16] Another demonstrated labeless immunoassay involves measuring the change in resistance on an electrode as antigens bind to it.
Counter-immunoelectrophoresis and its modification. In comparison to other conventional methods of diagnosis e.g. for viral infection testing, counter-immunoelectrophoresis is a highly specific, simple, and speedy method that does not require sophisticated, expensive tools, input materials, or long-term capacity building.
Ouchterlony double immunodiffusion, an immunological technique named after Örjan Ouchterlony Topics referred to by the same term This disambiguation page lists articles associated with the title Ochterlony .