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A 4Pi microscope is a laser scanning fluorescence microscope with an improved axial resolution.With it the typical range of the axial resolution of 500–700 nm can be improved to 100–150 nm, which corresponds to an almost spherical focal spot with 5–7 times less volume than that of standard confocal microscopy.
It is used in three dimensional geometry, and is analogous to the radian, which quantifies planar angles. A solid angle in the form of a right circular cone can be projected onto a sphere, defining a spherical cap where the cone intersects the sphere. The magnitude of the solid angle expressed in steradians is defined as the quotient of the ...
IBM System/4 Pi, a family of avionics computers; 4Pi microscope, a microscope that uses interference and fluorescence computers; 4×π = 12.56637..., the solid angle of a complete sphere measured in steradians; Hermetic detector (also called a 4π detector)
The solid angle of a sphere measured from any point in its interior is 4 π sr. The solid angle subtended at the center of a cube by one of its faces is one-sixth of that, or 2 π /3 sr. The solid angle subtended at the corner of a cube (an octant) or spanned by a spherical octant is π /2 sr, one-eight of the solid angle of a sphere. [1]
The number π (/ p aɪ / ⓘ; spelled out as "pi") is a mathematical constant, approximately equal to 3.14159, that is the ratio of a circle's circumference to its diameter.It appears in many formulae across mathematics and physics, and some of these formulae are commonly used for defining π, to avoid relying on the definition of the length of a curve.
In geometry, a simplex (plural: simplexes or simplices) is a generalization of the notion of a triangle or tetrahedron to arbitrary dimensions. The simplex is so-named because it represents the simplest possible polytope in any given dimension.
where C is the circumference of a circle, d is the diameter, and r is the radius.More generally, = where L and w are, respectively, the perimeter and the width of any curve of constant width.
The optimal interaction geometry places the cation in van der Waals contact with the aromatic ring, centered on top of the π face along the 6-fold axis. [3] Studies have shown that electrostatics dominate interactions in simple systems, and relative binding energies correlate well with electrostatic potential energy. [4] [5]