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To do a toeprint assay, one needs the mRNA of interest, ribosomes, a DNA primer, free nucleotides, and reverse transcriptase (RT), among other reagents. [4] The assay involves letting the RT generate cDNA until it gets blocked by any bound ribosomes, resulting in shorter fragments called toeprints when the results are observed on a sequencing gel .
The DNA template labeled at the 3' or 5' end, depending on the location of the binding site(s). Labels that can be used are: radioactivity and fluorescence . Radioactivity has been traditionally used to label DNA fragments for footprinting analysis, as the method was originally developed from the Maxam-Gilbert chemical sequencing technique.
In modern browsers, the print function of the browser should automatically use the rules in the style sheets when you print an article, therefore the print command of your web browser is also useful. Certain page elements normally do not print; these include self references like section edit links, navigation boxes, message boxes and metadata. [1]
Template documentation This template's initial visibility currently defaults to autocollapse , meaning that if there is another collapsible item on the page (a navbox, sidebar , or table with the collapsible attribute ), it is hidden apart from its title bar; if not, it is fully visible.
Print/export Download as PDF; Printable version; In other projects Wikidata item; ... Head to Toe may refer to: Head to Toe, a 1994 EP by the Breeders
Used to link tic tac toe and its variants Template parameters [Edit template data] This template prefers block formatting of parameters. Parameter Description Type Status state state This parameter may be used to set the template's initial visibility: if "collapsed" the content is hidden and only the title bar is visible (with [show] link), if "expanded" the content and title bar are fully ...
The PNG (Portable Network Graphics) file format was created as a free, open-source alternative to GIF. The PNG file format supports 8-bit (256 colors) paletted images (with optional transparency for all palette colors) and 24-bit truecolor (16 million colors) or 48-bit truecolor with and without alpha channel – while GIF supports only 8-bit ...
Tacticity (from Greek: τακτικός, romanized: taktikos, "relating to arrangement or order") is the relative stereochemistry of adjacent chiral centers within a macromolecule.