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A solution of phenol red is used as a pH indicator, often in cell culture. Its color exhibits a gradual transition from yellow (λ max = 443 nm [2]) to red (λ max = 570 nm [3]) over the pH range 6.8 to 8.2. Above pH 8.2, phenol red turns a bright pink color. [4] [5]
For example, phenol red exhibits an orange color between pH 6.8 and pH 8.4. The transition range may shift slightly depending on the concentration of the indicator in the solution and on the temperature at which it is used.
It is advised to check the references for photos of reaction results. [1] Reagent testers might show the colour of the desired substance while not showing a different colour for a more dangerous additive. [2]
A roll of universal indicator pape Colors of universal indicator. A universal indicator is a pH indicator made of a solution of several compounds that exhibit various smooth colour changes over a wide range pH values to indicate the acidity or alkalinity of solutions. A universal indicator can be in paper form or present in a form of a solution ...
The doubly deprotonated (In 2-) phenolate form (the anion form of phenol) gives the familiar pink color. In strongly basic solutions, phenolphthalein is converted to its In(OH) 3− form, and its pink color undergoes a rather slow fading reaction [6] and becomes completely colorless when pH is greater than 13.
Chlorophenol red is an indicator dye that changes color from yellow to violet in the pH range 5.4 to 6.8. [2] The pH of a substance is determined by taking the negative logarithm of the Hydronium ion concentration and the indictor changes color due to the dissociation of H + ions. [3] The lambda max is at 572 nm. [4]
DTM plate on the right showing color change after two days' growth; Microsporum canis Dermatophyte test medium (DTM) is a specialized agar used in medical mycology. [1] [2] It is based on Sabouraud's dextrose agar with added cycloheximide to inhibit saprotrophic growth, antibiotic to inhibit bacterial growth, and phenol red a pH indicator.
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