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The "dilution factor" is an expression which describes the ratio of the aliquot volume to the final volume. Dilution factor is a notation often used in commercial assays. For example, in solution with a 1/5 dilution factor (which may be abbreviated as x5 dilution ), entails combining 1 unit volume of solute (the material to be diluted) with ...
Three plates are needed for each dilution series, for statistical reasons an average of at least 3 counts are needed. The surface of the plates need to be sufficiently dry to allow a 20μl drop to be absorbed in 15–20 minutes. Plates are divided into equal sectors (it is possible to use up to 8 per plate).
V = air or gas volume of the closed space or room in cubic feet, cubic metres or litres; Q = ventilation rate into or out of the room in cubic feet per minute, cubic metres per hour or litres per second; C initial = initial concentration of a vapor inside the room measured in ppm; C final = final reduced concentration of the vapor inside the ...
A serial dilution is the step-wise dilution of a substance in solution, either by using a constant dilution factor, or by using a variable factor between dilutions. If the dilution factor at each step is constant, this results in a geometric progression of the concentration in a logarithmic fashion.
A viral titer is the lowest concentration of a virus that still infects cells. To determine the titer, several dilutions are prepared, such as 10 −1, 10 −2, 10 −3, ... 10 −8. [1] The titer of a fat is the temperature, in degrees Celsius, at which it solidifies. [4] The higher the titer, the harder the fat.
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A prior understanding of the microscopic anatomy of the organism can give a better understanding of how the observed CFU/mL relates to the number of viable cells per milliliter. Alternatively it is possible to decrease the average number of cells per CFU in some cases by vortexing the sample before conducting the dilution. However, many ...
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