Search results
Results from the WOW.Com Content Network
Imagine a book arranged like this: on all the right-hand pages, the text is printed as normal, but on the left hand pages, a different, unrelated text is printed upside down. You would consider that side to be the "non-reading side". But behold! rotate the book 180 degrees and now suddenly the "non-reading side" is the reading side and vice-versa.
Tl Dr: In the rosalind example they are showing the 3 reading frames that stem from the definition of a reading frame(non-overlapping triplets), not an example of open reading frames. Open reading frames are non-overlapping triplets between a start codon and a stop codon. Its important to step back and understand what is meant by reading frame ...
These are called reading frames. The Coding Sequence (CDS) is the actual region of DNA that is translated to form proteins. While the ORF may contain introns as well, the CDS refers to those nucleotides (concatenated exons) that can be divided into codons which are actually translated into amino acids by the ribosomal translation machinery.
By these criteria, reading frames F1, F2, F3 and F6, above are completely open (even though the internal methionine could theoretically be an initiation codon), F5 contains the open reading frame GAHGAGG, and F4 is a complete open reading frame, perhaps excluding the termination codon (depending on your semantic definition of the precise end of ...
My blanks and negative controls are averaging about 0.041 absorbance units (AU) at 540 nm, with an average A 450 of about 0.065. However, an A 450 of 0.9 gives an A 540 of 0.051, 1.4 gives 0.6, 2.0 gives 0.73, and 3.5 gives about 0.14. The absorbance spectrum (Fig. 2) of the substrate appears to be quite minimal at 540 nm, so why am I seeing ...
7. Standing genetic variation is when there is more than one allele at locus in the population at the time-point in question. When an allele goes to fixation there is no standing genetic variation at the locus until new mutations occur. Loci where alleles are not fixed are described as having standing genetic variation.
Suggested reading: I highly recommend the Jensen article referenced above. I read it when I was first starting to work on comparative genomics and evolution and it is a wonderfully clear and succinct explanation of the terms. Some of the articles referenced therein are also worth a read: Koonin EV: An apology for orthologs - or brave new memes.
$\begingroup$ The reading frame of the first exon defines the reading frame for all subsequent exons since they are literally ligated together into one long coding sequence. If you have two exons, say AUGGUA and AGUUGA, they are spliced together and translated to get the peptide Met-Val-Ser.
Most preinitiation complexes undergo translation initiation when they encounter an AUG start codon whether it is an efficient or inefficient context because the strong interaction between the codon and anticodon provide enough energy to drive the conformational shift. However, mismatches between a non-AUG start codon and the anticodon reduce ...
I agree that the definitions can be somewhat confusing when first encountered. Each nucleotide has a complement A-T, C-G. But the DNA strand are reverse-complementary because when aligned from 5'->3' they are not (necessarily) complementary. For example: DNA strand 5'-ATCCGG-3'. complement 3'-TAGGCC-5'. reverse 5'-CCGGAT-3'.