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Overlay of the two images is thus performed automatically as a result of the integration of two microscopes. [1] This technique is used in order to obtain information at different length scales: the electron microscope provides high-resolution information down to the nano-scale, while the fluorescence microscope highlights the regions of interest.
In a Kerr microscope, the illuminating light is first passed through a polarizer filter, then reflects from the sample and passes through an analyzer polarizing filter, before going through a regular optical microscope. Because the different MOKE geometries require different polarized light, the polarizer should have the option to change the ...
When light is transmitted through a layer of magneto-optic material, the result is called the Faraday effect: the plane of polarization can be rotated, forming a Faraday rotator. The results of reflection from a magneto-optic material are known as the magneto-optic Kerr effect (not to be confused with the nonlinear Kerr effect).
The optical microscope, also referred to as a light microscope, is a type of microscope that commonly uses visible light and a system of lenses to generate magnified images of small objects. Optical microscopes are the oldest design of microscope and were possibly invented in their present compound form in the 17th century.
By 1978, the first theoretical ideas had been developed to break the Abbe limit, which called for using a 4Pi microscope as a confocal laser-scanning fluorescence microscope where the light is focused from all sides to a common focus that is used to scan the object by 'point-by-point' excitation combined with 'point-by-point' detection. [14]
However, the physics of electron scattering and electron microscope image formation are sufficiently well known to allow accurate simulation of electron microscope images. [5] As a result of the interaction with a crystalline sample, the electron exit wave right below the sample φ e (x,u) as a function of the spatial coordinate x is a ...
This may be related to other properties of the object through the Beer–Lambert law. Precise measurements of the absorbance at many wavelengths allow the identification of a substance via absorption spectroscopy , where a sample is illuminated from one side, and the intensity of the light that exits from the sample in every direction is measured.
When the imaging system obeys the Abbe sine condition, the ratio of the sines of these angles equal the (lateral absolute) magnification of the system. In optics , the Abbe sine condition is a condition that must be fulfilled by a lens or other optical system in order for it to produce sharp images of off-axis as well as on-axis objects.