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It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article, RT-PCR will denote Reverse ...
The induction phase includes exposing a test group of animals twice to the test material, first by intradermal injection followed by topical application seven days later. During Induction A, the test animals are exposed intradermally to the test material, along with an adjuvant to enhance the immune reaction of the guinea pig. During Induction ...
A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...
The guinea pig or domestic guinea pig (Cavia porcellus), also known as the cavy or domestic cavy (/ ˈ k eɪ v i / KAY-vee), is a species of rodent belonging to the genus Cavia, family Caviidae. Breeders tend to use the name "cavy" for the animal, but "guinea pig" is more commonly used in scientific and laboratory contexts. [ 1 ]
The RT-LAMP technique is being supported as a cheaper and easier alternative to RT-PCR for the early diagnostics of people that are infectious for COVID-19. [6] There are open access test designs (including the recombinant proteins ) which makes it legally possible for anyone to produce a test.
Guinea pigs are easy to handle (once you know how) Guinea pig playing with a toy Every animal is different – that’s what makes them so special – but most guinea pigs love some human attention.
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Currently, rodents are commonly used in animal testing for physiological, pathological and behavioral scientific studies, particularly mice and rats, but also guinea pigs, hamsters, gerbils and others. Mice are the most commonly used vertebrate species, due to their availability, size, low cost, ease of handling, and fast reproduction rate