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An analytical profile index is a fast identification system based on biochemical incubation tests. Usually, this test is used to quickly diagnose clinically relevant bacteria by allowing physicians to run about 20 tests at one time. [19]
Indole test positive: appearance of pink layer at top (e.g. Escherichia coli) Like many biochemical tests on bacteria, results of an indole test are indicated by a change in color following a reaction with an added reagent. Pure bacterial culture must be grown in sterile tryptophan or peptone broth for 24–48 hours before performing the test.
Historically, biochemical tests have been an important tool used to discriminate between Staphylococcus species. [20] [8] Tests used to identify S. pseudintermedius specifically include DNase, [13] hyaluronidase, [22] coagulase, catalase, and acetoin production tests, amongst others. [8]
The IMViC tests are a group of individual tests used in microbiology lab testing to identify an organism in the coliform group.A coliform is a gram negative, aerobic, or facultative anaerobic rod, which produces gas from lactose within 48 hours.
The analytical profile index, or API, is a classification system for bacteria based on biochemical tests. The system was developed to accelerate the speed of identifying clinically relevant bacteria. It can only be used to identify known species from an index. [1] The data obtained are phenotypic traits.
Fresh bacterial preparations should be used. After colonies have grown on the medium, 2-3 drops of the reagent DMPD are added to the surface of each organism to be tested. A positive test (OX+) will result in a color change violet to purple, within 10–30 seconds. A negative test (OX-) will result in a light-pink or absence of coloration.
The TSI slant is a test tube that contains agar, a pH-sensitive dye , 1% lactose, 1% sucrose, 0.1% glucose, [2] and sodium thiosulfate and ferrous sulfate or ferrous ammonium sulfate. All of these ingredients are mixed together, heated to sterility, and allowed to solidify in the test tube at a slanted angle.
A bioassay is a biochemical test to estimate the potency of a sample compound. Usually this potency can only be measured relative to a standard compound. [3] [1] A typical bioassay involves a stimulus (ex. drugs) applied to a subject (ex. animals, tissues, plants). The corresponding response (ex. death) of the subject is thereby triggered and ...
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