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  2. 15 Most Common Puppy Health Issues and How to Spot Them - AOL

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    Comparative efficacy of two fipronil spot-on formulations against experimental tick infestations (Ixodes ricinus) in dogs. Parasitol Res. 2010 Aug;107(3):735-9. doi: 10.1007/s00436-010-1930-y ...

  3. Sticky and blunt ends - Wikipedia

    en.wikipedia.org/wiki/Sticky_and_blunt_ends

    The simplest DNA end of a double stranded molecule is called a blunt end. Blunt ends are also known as non-cohesive ends. In a blunt-ended molecule, both strands terminate in a base pair. Blunt ends are not always desired in biotechnology since when using a DNA ligase to join two molecules into one, the yield is significantly lower with blunt ...

  4. T7 DNA polymerase - Wikipedia

    en.wikipedia.org/wiki/T7_DNA_polymerase

    Figure 2.Nucleotidyl transfer by DNA polymerase. T7 DNA polymerase catalyzes the phosphoryl transfer [7] during DNA replication of the T7 phage.As shown in Figure 2, the 3’ hydroxyl group of a primer acts as a nucleophile and attacks the phosphodiester bond of nucleoside 5’-triphosphate (dTMP-PP).

  5. Cloning vector - Wikipedia

    en.wikipedia.org/wiki/Cloning_vector

    Schematic representation of the pBR322 plasmid, one of the first plasmids widely used as a cloning vector.. A cloning vector is a small piece of DNA that can be stably maintained in an organism, and into which a foreign DNA fragment can be inserted for cloning purposes. [1]

  6. Why Is My Senior Dog Peeing in the House? 7 Possible ... - AOL

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    Dogs with a bacterial infection feel an increased need to urinate. Some dogs will have dark urine or other signs of illness and, when treated with antibiotics and other treatments, this can clear ...

  7. Telltale Signs You Need to See a Doctor for Your Cough - AOL

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    For the record: It’s common for coughs to last for a while—even for months, according to Fady Youssef, M.D., a board-certified pulmonologist, internist and critical care specialist at ...

  8. Animal testing - Wikipedia

    en.wikipedia.org/wiki/Animal_testing

    The LD 50 ("Lethal Dose 50%") test is used to evaluate the toxicity of a substance by determining the dose required to kill 50% of the test animal population. This test was removed from OECD international guidelines in 2002, replaced by methods such as the fixed dose procedure, which use fewer animals and cause less suffering.

  9. Miami Beach doctor was charged with sex trafficking a minor ...

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    Gina was at the center of a sex trafficking case involving a prominent 67-year-old Miami Beach doctor she’d met on Tinder. ... as a medical consultant at a luxury drug treatment spa in Mexico ...