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  2. Sputum culture - Wikipedia

    en.wikipedia.org/wiki/Sputum_culture

    A sputum culture is a test to detect and identify bacteria [1] or fungi that infect the lungs or breathing passages. Sputum is a thick fluid produced in the lungs and in the adjacent airways. Normally, fresh morning sample is preferred for the bacteriological examination of sputum. [2]

  3. Sputum - Wikipedia

    en.wikipedia.org/wiki/Sputum

    The best sputum samples contain very little saliva, [2] as saliva contaminates the sample with oral bacteria. This is especially true for samples for laboratory testing in cytology or microbiology. Specimen adequacy is assessed by the laboratory technologists by examining a Gram stain or cytology stain of the sputum.

  4. Bronchoalveolar lavage - Wikipedia

    en.wikipedia.org/wiki/Bronchoalveolar_lavage

    Bronchoalveolar lavage (BAL), also known as bronchoalveolar washing, is a diagnostic method of the lower respiratory system in which a bronchoscope is passed through the mouth or nose into an appropriate airway in the lungs, with a measured amount of fluid introduced and then collected for examination.

  5. Diagnosis of tuberculosis - Wikipedia

    en.wikipedia.org/wiki/Diagnosis_of_tuberculosis

    The blood tests QuantiFERON-TB Gold In-Tube and T-SPOT.TB use these antigens to detect people with tuberculosis. Lymphocytes from the patient's blood are incubated with the antigens. These tests are called interferon γ tests and are not equivalent. [19] If the patient has been exposed to tuberculosis before, T lymphocytes produce interferon γ ...

  6. Diagnostic microbiology - Wikipedia

    en.wikipedia.org/wiki/Diagnostic_Microbiology

    The catalase test tests whether a microbe produces the enzyme catalase, which catalyzes the breakdown of hydrogen peroxide. Smearing a colony sample onto a glass slide and adding a solution of hydrogen peroxide (3% H 2 O 2) will indicate whether the enzyme is present or not. Bubbling is a positive test while nothing happening is a negative ...

  7. Ziehl–Neelsen stain - Wikipedia

    en.wikipedia.org/wiki/Ziehl–Neelsen_stain

    The acidity of the dyes causes them to bind more strongly to the cell walls of the bacteria than to other cells or tissues. This results in the selective staining of only those cells that have a high density of cell wall material, such as acid-fast bacteria. [26] The Ziehl-Neelsen stain is a two step staining process. In the first step, the ...

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