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Fluorescence in several wavelengths can be detected by an array detector, to detect compounds from HPLC flow. Also, TLC plates can be visualized if the compounds or a coloring reagent is fluorescent. Fluorescence is most effective when there is a larger ratio of atoms at lower energy levels in a Boltzmann distribution. There is, then, a higher ...
A simplified Jablonski diagram illustrating the change of energy levels.. The principle behind fluorescence is that the fluorescent moiety contains electrons which can absorb a photon and briefly enter an excited state before either dispersing the energy non-radiatively or emitting it as a photon, but with a lower energy, i.e., at a longer wavelength (wavelength and energy are inversely ...
Fluorescence is the emission of light by a molecule or an atom that has absorbed light or other electromagnetic radiation. In most cases, the emitted light has a longer wavelength , and therefore a lower photon energy , than the absorbed radiation.
X-ray fluorescence (XRF) is the emission of characteristic "secondary" (or fluorescent) ... being greatest when mean atomic number is low. When measuring trace ...
Fluorescence spectroscopy (also known as fluorimetry or spectrofluorometry) is a type of electromagnetic spectroscopy that analyzes fluorescence from a sample. It involves using a beam of light, usually ultraviolet light , that excites the electrons in molecules of certain compounds and causes them to emit light; typically, but not necessarily ...
The fluorescence of certain rocks and other substances had been observed for hundreds of years before its nature was understood. One of the first to explain it was Irish scientist Sir George Stokes from the University of Cambridge in 1852, who named the phenomenon "fluorescence" after fluorite, a mineral many of whose samples glow strongly because of impurities.
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Photoluminescence processes can be classified by various parameters such as the energy of the exciting photon with respect to the emission. Resonant excitation describes a situation in which photons of a particular wavelength are absorbed and equivalent photons are very rapidly re-emitted. This is often referred to as resonance fluorescence.