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  2. Umu Chromotest - Wikipedia

    en.wikipedia.org/wiki/Umu_Chromotest

    The degree of color development is an indirect measure of the β-galactosidase produced, which itself is directly related to the amount of DNA damage. The Umu Chromotest has the added advantage of having its procedure codified under ISO 13829 "Water Quality- Determination of genotoxicity of water and waste water using the umu-test".

  3. SOS chromotest - Wikipedia

    en.wikipedia.org/wiki/SOS_Chromotest

    Overview of the use of the SOS response for genotoxicity testing. The SOS chromotest is a biological assay to assess the genotoxic potential of chemical compounds. The test is a colorimetric assay which measures the expression of genes induced by genotoxic agents in Escherichia coli, by means of a fusion with the structural gene for β-galactosidase.

  4. β-Galactosidase - Wikipedia

    en.wikipedia.org/wiki/Β-Galactosidase

    β-Galactosidase (EC 3.2.1.23, beta-gal or β-gal; systematic name β-D-galactoside galactohydrolase) is a glycoside hydrolase enzyme that catalyzes hydrolysis of terminal non-reducing β-D-galactose residues in β-D-galactosides. (This enzyme digests many β-Galactosides, not just lactose.

  5. ortho-Nitrophenyl-β-galactoside - Wikipedia

    en.wikipedia.org/wiki/Ortho-Nitrophenyl-β...

    ortho-Nitrophenyl-β-galactoside (ONPG) is a colorimetric and spectrophotometric substrate for detection of β-galactosidase activity. [1] This compound is normally colorless. However, if β-galactosidase is present, it hydrolyzes the ONPG molecule into galactose and ortho-nitrophen

  6. Galactosialidosis - Wikipedia

    en.wikipedia.org/wiki/Galactosialidosis

    Galactosialidosis occurs when a patient inherits two copies of a mutated CTSA gene. Encoding of the mutated gene results in a defective form of the protein cathepsin A. When the structure of cathepsin A is disrupted due to mutation, it becomes non-functional and cannot form a digestive complex with neuraminidase-1 and beta-galactosidase.

  7. Galactosidases - Wikipedia

    en.wikipedia.org/wiki/Galactosidases

    Galactosidases are enzymes (glycoside hydrolases) that catalyze the hydrolysis of galactosides into monosaccharides.. Galactosides can be classified as either alpha or beta. If the galactoside is classified as an alpha-galactoside, the enzyme is called alpha-galactosidase, and is responsible for catalyzing the hydrolysis of substrates that contain α-galactosidic residues, such as ...

  8. GLB1 - Wikipedia

    en.wikipedia.org/wiki/GLB1

    The RNA transcript of the GLB1 gene is alternatively spliced and produces 2 mRNAs. The 2.5-kilobase transcript encodes the beta-galactosidase enzyme of 677 amino acids.The alternative 2.0-kb mRNA encodes a beta-galactosidase-related protein (S-Gal) that is only 546 amino acids long and that has no enzymatic activity.

  9. GM1 gangliosidoses - Wikipedia

    en.wikipedia.org/wiki/GM1_gangliosidoses

    The GM1 gangliosidoses, usually shortened to GM1, are gangliosidoses caused by mutation in the GLB1 gene resulting in a deficiency of beta-galactosidase.The deficiency causes abnormal storage of acidic lipid materials in cells of the central and peripheral nervous systems, but particularly in the nerve cells, resulting in progressive neurodegeneration.

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