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These are considered heterophile antibodies. In clinical diagnosis, the heterophile antibody test specifically refers to a rapid test for antibodies produced against the Epstein-Barr virus (EBV), the causative agent of infectious mononucleosis. Heterophile antibodies can cause significant interference in any immunoassay. [3]
The mononuclear spot test or monospot test, a form of the heterophile antibody test, [1] is a rapid test for infectious mononucleosis due to Epstein–Barr virus (EBV). It is an improvement on the Paul–Bunnell test. [2] The test is specific for heterophile antibodies produced by the human immune system in response to EBV
False positive pregnancy test can happen due to 'phantom hCG' which is due to people having human antianimal or heterophilic antibodies. [21] False positives can also be caused by (in order of incidence) quiescent pregnancy, pituitary sulfated hCG, heterophilic antibody, familial hCG syndrome and cancer. [22]
Antibody to EBNA slowly appears 2 to 4 months after the onset of symptoms and persists for the rest of a person’s life. [10] When negative, these tests are more accurate than the heterophile antibody test in ruling out infectious mononucleosis. When positive, they feature similar specificity to the heterophile antibody test.
Other heterophile antigens are responsible for some diagnostic serological tests such as: Weil-Felix reaction for typhus fever; Paul Bunnell test for infectious mononucleosis; Cold agglutinin test in primary atypical pneumonia; Chemically, heterophile antigens are composed of lipoprotein-polysaccharide complexes.
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