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  2. Patch clamp - Wikipedia

    en.wikipedia.org/wiki/Patch_clamp

    For some types of cells, it is convenient to apply both methods simultaneously to break the patch. The advantage of whole-cell patch clamp recording over sharp electrode technique recording is that the larger opening at the tip of the patch clamp electrode provides lower resistance and thus better electrical access to the inside of the cell.

  3. Automated patch clamp - Wikipedia

    en.wikipedia.org/wiki/Automated_patch_clamp

    A schematic of a patch clamp chip showing a gigaseal, whole cell recording configuration, and the ion channel and whole cell currents. Many types of systems have been developed for patch clamping cells in suspension cultures. One system uses a traditional pipette and cells in a droplet suspension culture to obtain patch clamp recordings (see ...

  4. Patch-sequencing - Wikipedia

    en.wikipedia.org/wiki/Patch-Sequencing

    The patch pipette is designed for whole cell recording so its opening diameter is larger than experiments done to examine single ion-channels. For the most part standard patch clamp protocols may be used although there are some small situation dependent modifications to the pipette and the internal solution.

  5. Glucose clamp technique - Wikipedia

    en.wikipedia.org/wiki/Glucose_clamp_technique

    Hyperglycemic clamp technique: The plasma glucose concentration is acutely raised to 125 mg/dl above basal levels by a continuous infusion of glucose. This hyperglycemic plateau is maintained by adjustment of a variable glucose infusion, based on the rate of insulin secretion and glucose metabolism .

  6. Voltage clamp - Wikipedia

    en.wikipedia.org/wiki/Voltage_clamp

    The voltage clamp is an experimental method used by electrophysiologists to measure the ion currents through the membranes of excitable cells, such as neurons, while holding the membrane voltage at a set level. [1] A basic voltage clamp will iteratively measure the membrane potential, and then change the membrane potential (voltage) to a ...

  7. Optogenetics - Wikipedia

    en.wikipedia.org/wiki/Optogenetics

    Fig 1. Channelrhodopsin-2 (ChR2) induces temporally precise blue light-driven activity in rat prelimbic prefrontal cortical neurons. a) In vitro schematic (left) showing blue light delivery and whole-cell patch-clamp recording of light-evoked activity from a fluorescent CaMKllα::ChR2-EYFP expressing pyramidal neuron (right) in an acute brain slice.

  8. Roberts was arrested for allegedly failing to appear in court on charges of offering to engage in an act of prostitution and assault with a dangerous weapon, according to Fox 25. Roberts was found dead in her cell. Jail or Agency: Oklahoma County Jail; State: Oklahoma; Date arrested or booked: 3/23/2016; Date of death: 4/11/2016; Age at death: 59

  9. Smart insulin patch - Wikipedia

    en.wikipedia.org/wiki/Smart_Insulin_Patch

    Researchers first reported the concept of "smart insulin patch" in 2015. [1] The prototype of smart insulin patch "was demonstrated as a continuous glucose control in a type 1 diabetic mouse model. [1] [2] [3] As of 2019, glucose-responsive insulin patches are becoming more common. [4] In 2020, scientists at UCLA and Zenomics Inc. developed ...

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