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The 5-day test protocol with acceptably reproducible results emphasizing carbonaceous BOD has been endorsed by the United States Environmental Protection Agency (EPA). This 5-day BOD test result may be described as the amount of oxygen required for aquatic microorganisms to stabilize decomposable organic matter under aerobic conditions. [ 7 ]
Both have been widely adopted as a measure of pollution effect. The BOD test measures the oxygen demand of biodegradable pollutants whereas the COD test measures the oxygen demand of oxidizable pollutants. The so-called 5-day BOD measures the amount of oxygen consumed by biochemical oxidation of waste contaminants in a 5-day period.
BOD Bottle BOD test bottles at the laboratory of a wastewater treatment plant. BOD Bottle or an incubation bottle is a main apparatus used for the Biological Oxygen Demand (BOD) test. During the five-day BOD or BOD5 test process, the BOD bottle is used for incubating diluted samples under the 20 °C or 68 °F of temperature.
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The difference in DO and the dilution factor are used to calculated BOD 5. The resulting number (usually reported in parts per million or milligrams per liter) is useful in determining the relative organic strength of sewage or other polluted waters. The BOD 5 test is an example of analysis that determines classes of materials in a sample.
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In environmental chemistry, the chemical oxygen demand (COD) is an indicative measure of the amount of oxygen that can be consumed by reactions in a measured solution.It is commonly expressed in mass of oxygen consumed over volume of solution, which in SI units is milligrams per liter (mg/L).
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