Search results
Results from the WOW.Com Content Network
Many single-cell analysis techniques require the isolation of individual cells. Methods currently used for single-cell isolation include: dielectrophoretic digital sorting, enzymatic digestion, FACS, hydrodynamic traps, laser capture microdissection, manual picking, microfluidics, Inkjet Printing (IJP), micromanipulation, serial dilution, and Raman tweezers.
Plant tissue culture is a collection of techniques used to maintain or grow plant cells, tissues, or organs under sterile conditions on a nutrient culture medium of known composition. It is widely used to produce clones of a plant in a method known as micropropagation .
Cell isolation is the process of separating individual living cells from a solid block of tissue or cell suspension. While some types of cell naturally exist in a separated form (for example blood cells), other cell types that are found in solid tissue require specific techniques to separate them into individual cells.
Laser-capture microdissection (LCM) is a method to procure subpopulations of tissue cells under direct microscopic visualization. LCM technology can harvest the cells of interest directly or can isolate specific cells by cutting away unwanted cells to give histologically pure enriched cell populations.
Single-nucleotide polymorphisms (SNPs), which are a big part of genetic variation in the human genome, and copy number variation (CNV), pose problems in single cell sequencing, as well as the limited amount of DNA extracted from a single cell. Due to scant amounts of DNA, accurate analysis of DNA poses problems even after amplification since ...
The cost of growing mammalian cell cultures is high, so research is underway to produce such complex proteins in insect cells or in higher plants, use of single embryonic cell and somatic embryos as a source for direct gene transfer via particle bombardment, transit gene expression and confocal microscopy observation is one of its applications ...
A. tumefaciens attaching itself to a carrot cell. In plants the DNA is often inserted using Agrobacterium-mediated recombination, [27] taking advantage of the Agrobacteriums T-DNA sequence that allows natural insertion of genetic material into plant cells. [28] Plant tissue are cut into small pieces and soaked in a fluid containing suspended ...
CHO cells in suspension. A cell suspension or suspension culture is a type of cell culture in which single cells or small aggregates of cells are allowed to function and multiply in an agitated growth medium, thus forming a suspension. Suspension culture is one of the two classical types of cell culture, the other being adherent culture. The ...