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2. Oil immersion - Wikipedia

   en.wikipedia.org/wiki/Oil_immersion

   Oil-immersion objective lenses look superficially identical to non-oil-immersion lenses. In light microscopy , oil immersion is a technique used to increase the resolving power of a microscope . This is achieved by immersing both the objective lens and the specimen in a transparent oil of high refractive index , thereby increasing the numerical ...

3. Objective (optics) - Wikipedia

   en.wikipedia.org/wiki/Objective_(optics)

   Two Leica oil immersion microscope objective lenses; left 100×, right 40×. The objective lens of a microscope is the one at the bottom near the sample. At its simplest, it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes ...

4. Optical microscope - Wikipedia

   en.wikipedia.org/wiki/Optical_microscope

   An oil immersion lens usually has a magnification of 40 to 100×. ... For example a 10x eyepiece magnification and a 100x objective lens magnification gives a total ...

5. Wikipedia : Picture peer review/Microscope Objective Lens

   en.wikipedia.org/.../Microscope_Objective_Lens

   Original - Two Leica oil immersion microscope objective lenses; left 100x, right 40x. This is a high quality image of an object, a microscope objective lens, most people would otherwise not get a chance to see. Articles this image appears in Optical microscope Objective (optics) Oil immersion Creator Richard Wheeler Suggested by

6. Numerical aperture - Wikipedia

   en.wikipedia.org/wiki/Numerical_aperture

   where n is the index of refraction of the medium in which the lens is working (1.00 for air, 1.33 for pure water, and typically 1.52 for immersion oil; [1] see also list of refractive indices), and θ is the half-angle of the maximum cone of light that can enter or exit the lens.

7. Magnification - Wikipedia

   en.wikipedia.org/wiki/Magnification

   With an optical microscope having a high numerical aperture and using oil immersion, the best possible resolution is 200 nm corresponding to a magnification of around 1200×. Without oil immersion, the maximum usable magnification is around 800×. For details, see limitations of optical microscopes.

8. Index-matching material - Wikipedia

   en.wikipedia.org/wiki/Index-matching_material

   An oil immersion objective is an objective lens specially designed to be used in this way. The index of the oil is typically chosen to match the index of the microscope lens glass, and of the cover slip. For more details, see the main article, oil immersion.

9. Live-cell imaging - Wikipedia

   en.wikipedia.org/wiki/Live-cell_imaging

   Oil immersion is a technique that can increase image resolution by immersing the lens and the specimen in oil with a high refractive index. Since light bends when it passes between media with different refractive indexes, by placing oil with the same refractive index as glass between the lens and the slide, two transitions between refractive ...