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P1 is a temperate bacteriophage that infects Escherichia coli and some other bacteria. When undergoing a lysogenic cycle the phage genome exists as a plasmid in the bacterium [1] unlike other phages (e.g. the lambda phage) that integrate into the host DNA. P1 has an icosahedral head containing the DNA attached to a contractile tail with six ...
The p1 protein of Ff phage (i. e. genus Inovirus), which is required for phage assembly at the membrane, has a membrane-spanning hydrophobic domain with the N-terminal portion in the cytoplasm and the C-terminal portion in the periplasm (the reverse of the orientation of the gene 8 coat protein). Adjacent to the cytoplasmic side of the membrane ...
The p1 protein contains Walker motifs which are essential for phage assembly, [24] suggesting that p1 is a molecular motor involved in phage assembly. The p1 protein has a membrane-spanning hydrophobic domain with the N-terminal portion in the cytoplasm and the C-terminal portion in the periplasm (the reverse of the orientation of p8).
Enterobacteria phage λ (lambda phage, coliphage λ, officially Escherichia virus Lambda) is a bacterial virus, or bacteriophage, that infects the bacterial species Escherichia coli (E. coli). It was discovered by Esther Lederberg in 1950. [ 2 ]
Bacteriophage P2 was first isolated by G. Bertani from the Lisbonne and Carrère strain of E. coli in 1951. [3] Since that time, a large number of P2-like prophages (e.g. 186, HP1, HK239, and WΦ) have been isolated that shared characters such as host range, serological relatedness and inability to recombine with phage λ, and they seemed to be quite common in E. coli populations as about 30% ...
M13 is one of the Ff phages (fd and f1 are others), a member of the family filamentous bacteriophage ().Ff phages are composed of circular single-stranded DNA (), which in the case of the m13 phage is 6407 nucleotides long and is encapsulated in approximately 2700 copies of the major coat protein p8, and capped with about 5 copies each of four different minor coat proteins (p3 and p6 at one ...
Escherichia coli (E. coli), common Gram-negative gut bacterium widely used in molecular genetics. Main lab strain is 'K-12'. Bacillus subtilis, endospore forming Gram-positive bacterium. Main lab strain is '168'. Caulobacter crescentus, bacterium that divides into two distinct cells used to study cellular differentiation.
The virus attaches to the host cell lipopolysaccharides using its terminal fibers, and ejects the viral DNA into the host cytoplasm via contraction of its tail sheath. Once in the cell, the viral DNA is protected from degradation by the host nucleases.