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  2. Lysis buffer - Wikipedia

    en.wikipedia.org/wiki/Lysis_buffer

    RIPA buffer is a commonly used lysis buffer for immunoprecipitation and general protein extraction from cells and tissues. The buffer can be stored without vanadate at 4 °C for up to 1 year. [10] RIPA buffer releases proteins from cells as well as disrupts most weak interactions between proteins. [9] Recipe: [10] 1% (w/w) Nonidet P-40 (NP-40)

  3. DNA extraction - Wikipedia

    en.wikipedia.org/wiki/DNA_extraction

    The purified nuclei are then lysed and further cleaned by organic extraction, and the genomic DNA is precipitated with a high concentration of CTAB. The highly pure, high molecular weight gDNA is extracted from the nuclei, dissolved in a high pH buffer, allowing for stable long-term storage.

  4. Spin column-based nucleic acid purification - Wikipedia

    en.wikipedia.org/wiki/Spin_column-based_nucleic...

    Silica in a spin column with water and with DNA sample in chaotropic buffer. Spin column-based nucleic acid purification is a solid phase extraction method to quickly purify nucleic acids. This method relies on the fact that nucleic acid will bind to the solid phase of silica under certain conditions.

  5. Miltenyi Biotec - Wikipedia

    en.wikipedia.org/wiki/Miltenyi_Biotec

    Miltenyi Biotec is a global biotechnology company headquartered near Cologne in Bergisch Gladbach, Germany. The company is a provider of products and services for scientists, clinical researchers, and physicians to use in their basic research, translational research , and clinical applications.

  6. Radioimmunoprecipitation assay buffer - Wikipedia

    en.wikipedia.org/wiki/Radioimmunoprecipitation...

    Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used to lyse cells and tissue for the radio immunoprecipitation assay (RIPA). [1] [2] This buffer is more denaturing than NP-40 or Triton X-100 because it contains the ionic detergents SDS and sodium deoxycholate as active constituents and is particularly useful for disruption of nuclear membranes in the preparation of ...

  7. DNA separation by silica adsorption - Wikipedia

    en.wikipedia.org/wiki/DNA_separation_by_silica...

    The highest DNA adsorption efficiencies occur in the presence of buffer solution with a pH at or below the pKa of the surface silanol groups. The mechanism behind DNA adsorption onto silica is not fully understood; one possible explanation involves reduction of the silica surface's negative charge due to the high ionic strength of the buffer.

  8. Trump squeeze wins concessions - some real, others less so - AOL

    www.aol.com/news/trump-squeeze-wins-concessions...

    Even more uncertain is what will come of Trump's assertion that the United States must get a share of revenues from the future extraction of Ukraine's deposits of rare earths and other critical ...

  9. Protein purification - Wikipedia

    en.wikipedia.org/wiki/Protein_purification

    The protein manufacturing cost remains high and there is a growing demand to develop cost efficient and rapid protein purification methods. Understanding the different protein purification methods and optimizing the downstream processing is critical to minimize production costs while maintaining the quality of acceptable standards of homogeneity. [2]