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A real-time polymerase chain reaction (real-time PCR, or qPCR when used quantitatively) is a laboratory technique of molecular biology based on the polymerase chain reaction (PCR). It monitors the amplification of a targeted DNA molecule during the PCR (i.e., in real time), not at its end, as in conventional PCR. Real-time PCR can be used ...
It is primarily used to measure the amount of a specific RNA. This is achieved by monitoring the amplification reaction using fluorescence, a technique called real-time PCR or quantitative PCR (qPCR). Confusion can arise because some authors use the acronym RT-PCR to denote real-time PCR. In this article, RT-PCR will denote Reverse ...
In January, Cosmos Health Inc. (NASDAQ:COSM) entered into a distribution agreement with Virax Biolabs to distribute Virax-branded Avian Influenza Virus Real-Time PCR Kits across various major ...
Truenat is a chip-based, point-of-care, rapid molecular test for diagnosis of infectious diseases. The technology is based on the Taqman RTPCR (Real Time Reverse Transcription Polymerase Chain Reaction) chemistry which can be performed on the portable, battery operated Truelab Real Time micro PCR platform.
Primerdesign was founded in 2005 by Dr Jim Wicks, Dr Rob Powell and Professor Tom Brown within the University of Southampton to focus on PCR and DNA chemistry. The company has grown since then and its products have been used in over 100 countries. The company has a portfolio of over 400 qPCR detection kits and over 9000 research targets. [1]
A quantitative PCR instrument [1] is a machine that amplifies and detects DNA. It combines the functions of a thermal cycler and a fluorimeter , enabling the process of quantitative PCR . The first quantitative PCR machine was described in 1993, [ 2 ] and two commercial models became available in 1996.
Quantitative PCR (Q-PCR) is used to measure the quantity of a PCR product (preferably real-time, QRT-PCR). [2] It is the method of choice to quantitatively measure amounts of transgene DNA in a food or feed sample. Q-PCR is commonly used to determine whether a DNA sequence is present in a sample and the number of its copies in the sample.
Seegene’s primary product focus is multiplex diagnostic assays, which allow for the detection and quantification of multiple pathogens from a single sample. [4] Seegene's assays are developed using its proprietary Dual Priming Oligonucleotide (DPO), Tagging Oligonucleotide Cleavage and Extension (TOCE), and Multiple Detection Temperatures (MuDT) technology platforms.
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