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"Ouchterlony double immunodiffusion" (photograph) permanent dead link ] Photograph of Ouchterlony double immunodiffusion plate with unstained precipitin lines of full identity and non-identity. "Diffusion Patterns". Immunodiffusion principles and application. Archived from the original on 2019-12-11
Immunodiffusion is a laboratory technique used to detect and quantify antigens and antibodies by observing their interactions within a gel medium. [1] This technique involves the diffusion of antigens and antibodies through a gel, usually agar, resulting in the formation of a visible precipitate when they interact.
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Surface plasmon resonance is an example of technique that can detect binding between an unlabeled antibody and antigens. [16] Another demonstrated labeless immunoassay involves measuring the change in resistance on an electrode as antigens bind to it.
A solution containing antibody is added to a heated medium such as agar or agarose dissolved in buffered normal saline.The molten medium is then poured onto a microscope slide or into an open container, such as a Petri dish, and allowed to cool and form a gel.
Counter-immunoelectrophoresis and its modification. In comparison to other conventional methods of diagnosis e.g. for viral infection testing, counter-immunoelectrophoresis is a highly specific, simple, and speedy method that does not require sophisticated, expensive tools, input materials, or long-term capacity building.
Örjan Thomas Ouchterlony (January 14, 1914, Stockholm – September 25, 2004) was a Swedish bacteriologist and immunologist who is credited with the creation of the Ouchterlony double immuno diffusion test in the 1940s. [1] [2] He was trained at Karolinska Institute, where his received his medical doctorate.
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