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Pyrosequencing is a method of DNA sequencing (determining the order of nucleotides in DNA) based on the "sequencing by synthesis" principle, in which the sequencing is performed by detecting the nucleotide incorporated by a DNA polymerase. Pyrosequencing relies on light detection based on a chain reaction when pyrophosphate is released. Hence ...
Standard flowgram format (SFF) is a binary file format used to encode results of pyrosequencing from the 454 Life Sciences platform for high-throughput sequencing.SFF files can be viewed, edited and converted with DNA Baser SFF Workbench (graphic tool), or converted to FASTQ format with sff2fastq or seq_crumbs.
In a de Bruijn graph, there is a possibility of 4^k different nodes to make arrangements of a genome. The number of nodes used to create the graph can be reduced in number by considering only the k-mers found within the DNA strand of interest. Given sequence 1, it is possible to determine the nodes of size 7, or 7-mers, that will be in the graph.
Phred quality scores shown on a DNA sequence trace. A Phred quality score is a measure of the quality of the identification of the nucleobases generated by automated DNA sequencing.
GraphCalc is an open-source computer program that runs in Microsoft Windows and Linux that provides the functionality of a graphing calculator. GraphCalc includes many of the standard features of graphing calculators, but also includes some higher-end features: High resolution
After reading the introduction I feel that the 454 should only be mentioned later under recent advantages. This way it appears that 454 is the only way pyrosequencing is applied. In addition, it is a bit of a repetition.CharonZ 10:06, 10 July 2007 (UTC)
The calculator also handles vectors, matrices and complex numbers better than the TI-83. One drawback, however, is that the statistics package on the TI-83 range doesn't come preloaded on the TI-86. However, it can be downloaded from the Texas Instruments program archive and installed on the calculator using the link cable. [1]
HP 48G calculator, uses RPL RPL [5] is a handheld calculator operating system and application programming language used on Hewlett-Packard 's scientific graphing RPN (Reverse Polish Notation) calculators of the HP 28 , 48 , 49 and 50 series, but it is also usable on non-RPN calculators, such as the 38 , 39 and 40 series.