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Mechanism of self-amplifying mRNA (saRNA) used for antigen production. The ORF encoding the antigen can also be substituted with a protein for use in protein replacement therapy. This sequence encodes the protein of interest, used as an antigen in the case of vaccines or for protein replacement therapies. The gene of interest replaces the viral ...
To identify an saRNA that can activate a gene of interest, several saRNAs need to be designed within a 1- to 2-kbp promoter region by following a set of rules [3] [4] and tested in cultured cells. In some reports, saRNAs are designed in such a way to target non-coding transcripts that overlap the promoter sequence of a protein coding gene.
Baked beans is a dish traditionally containing white common beans that are parboiled and then baked in sauce at low temperature for a lengthy period. [1] Canned baked beans are not baked, but are cooked through a steam process. [2] Baked beans originated in Native American cuisine, and are made from beans indigenous to the Americas. [3]
These are known variously as loaded potato skins, filled potatoes and twice-baked potatoes. In Great Britain, toppings or fillings include baked beans, curried chicken, coronation chicken, chili con carne, shredded cheese, tuna mayonnaise, and coleslaw. In Scotland even haggis is used as a filling for jacket potatoes.
Accidents in the world of food can occasionally lead to the discovery of something delicious, but most of the time cooking mistakes lead to undercooked roasts, spreading cookies and inedible eats.
Twice-Baked Potatoes. Twice-baked potatoes are basically a way to eat mashed potatoes in a cute little potato boat. The first bake is roasting the potatoes so they are nice and tender. While the ...
The most commonly used are dehydrated flaked mashed potatoes, or instant mashed potatoes, and pre-cooked frozen French fries. The latter are more commonly consumed in the catering industry. The simplest are peeled and pre-cooked vacuum-packed potatoes, which belong to the category of fresh products, known as fifth range.
The same principle applies when baking soda is used to boil pretzels before baking. The pantry staple can also serve as a tenderizer for certain ingredients—like meat and even canned chickpeas .