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[2] [10] The specifier sequence is the first recognition sequence in the leader. [7] It is complementary to the anticodon of the tRNA that is a substrate of the tRNA synthetase under regulation. [7] The second tRNA binding sequence, the T box sequence, is complementary to the nucleotide preceding the acceptor end of the tRNA. [7]
The 5′ untranslated region (also known as 5′ UTR, leader sequence, transcript leader, or leader RNA) is the region of a messenger RNA (mRNA) that is directly upstream from the initiation codon. This region is important for the regulation of translation of a transcript by differing mechanisms in viruses , prokaryotes and eukaryotes .
Trans-Spliced Exon Coupled RNA End Determination (TEC-RED) is a transcriptomic technique that, like SAGE, allows for the digital detection of messenger RNA sequences.Unlike SAGE, detection and purification of transcripts from the 5’ end of the messenger RNA require the presence of a trans-spliced leader sequence.
Leader sequence may refer to: Leader sequence (mRNA), a sequence of mRNA; Leading strand, in DNA replication This page was last edited on 28 ...
New spacers are added to a CRISPR array in a directional manner, [32] occurring preferentially, [81] [124] [125] [132] [133] but not exclusively, adjacent [127] [130] to the leader sequence. Analysis of the type I-E system from E. coli demonstrated that the first direct repeat adjacent to the leader sequence is copied, with the newly acquired ...
CRISPR RNA or crRNA is a RNA transcript from the CRISPR locus. [1] CRISPR-Cas (clustered, regularly interspaced short palindromic repeats - CRISPR associated systems) is an adaptive immune system found in bacteria and archaea to protect against mobile genetic elements, like viruses, plasmids, and transposons. [2] The CRISPR locus contains a ...
The target sequence is 20 bases long as part of each CRISPR locus in the crRNA array. [58] A typical crRNA array has multiple unique target sequences. Cas9 proteins select the correct location on the host's genome by utilizing the sequence to bond with base pairs on the host DNA.
[23] [46] The first step amplifies the sgRNA region, using primers specific to the lentiviral integration sequence, and the second step adds Illumina i5 and i7 sequences. [23] NGS of the PCR products allows the recovered sgRNAs to be identified, and a quantification step can be used to determine the relative abundance of each sgRNA.