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  2. cDNA library - Wikipedia

    en.wikipedia.org/wiki/CDNA_library

    A cDNA library is a combination of cloned cDNA (complementary DNA) fragments inserted into a collection of host cells, which constitute some portion of the transcriptome of the organism and are stored as a "library". cDNA is produced from fully transcribed mRNA found in the nucleus and therefore contains only the expressed genes of an organism.

  3. Library (biology) - Wikipedia

    en.wikipedia.org/wiki/Library_(biology)

    A cDNA library represents a sample of the mRNA purified from a particular source (either a collection of cells, a particular tissue, or an entire organism), which has been converted back to a DNA template by the use of the enzyme reverse transcriptase.

  4. Complementary DNA - Wikipedia

    en.wikipedia.org/wiki/Complementary_DNA

    RNA serves as a template for cDNA synthesis. [3] In cellular life, cDNA is generated by viruses and retrotransposons for integration of RNA into target genomic DNA.In molecular biology, RNA is purified from source material after genomic DNA, proteins and other cellular components are removed. cDNA is then synthesized through in vitro reverse transcription.

  5. Genomic library - Wikipedia

    en.wikipedia.org/wiki/Genomic_library

    A genomic library is a collection of overlapping DNA fragments that together make up the total genomic DNA of a single organism. The DNA is stored in a population of identical vectors , each containing a different insert of DNA.

  6. IMAGE cDNA clones - Wikipedia

    en.wikipedia.org/wiki/IMAGE_cDNA_clones

    IMAGE cDNA clones are a collection of DNA vectors containing cDNAs from various organisms including human, mouse, rat, non-human primates, zebrafish, pufferfish, Xenopus (frogs), and cow. [ 1 ] Together they represent a more or less complete set of expressed genes from these organisms.

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  8. Expressed sequence tag - Wikipedia

    en.wikipedia.org/wiki/Expressed_sequence_tag

    An EST results from one-shot sequencing of a cloned cDNA. The cDNAs used for EST generation are typically individual clones from a cDNA library. The resulting sequence is a relatively low-quality fragment whose length is limited by current technology to approximately 500 to 800 nucleotides. Because these clones consist of DNA that is ...

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