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  2. Genome-wide CRISPR-Cas9 knockout screens - Wikipedia

    en.wikipedia.org/wiki/Genome-wide_CRISPR-Cas9...

    In the specific context of genome-wide CRISPR screens, producing and transducing the lentiviral particles is relatively laborious and time-consuming, taking about two weeks in total. [44] Additionally, because the DNA integrates into the host genome, lentiviral delivery leads to long-term expression of Cas9, potentially leading to off-target ...

  3. Genetic engineering techniques - Wikipedia

    en.wikipedia.org/wiki/Genetic_engineering_techniques

    Genome editing uses artificially engineered nucleases that create specific double-stranded breaks at desired locations in the genome. The breaks are subject to cellular DNA repair processes that can be exploited for targeted gene knock-out, correction or insertion at high frequencies.

  4. Synthetic genetic array - Wikipedia

    en.wikipedia.org/wiki/Synthetic_genetic_array

    Synthetic genetic array analysis is generally conducted using colony arrays on petriplates at standard densities (96, 384, 768, 1536). To perform a SGA analysis in S.cerevisiae, the query gene deletion is crossed systematically with a deletion mutant array (DMA) containing every viable knockout ORF of the yeast genome (currently 4786 strains). [9]

  5. Genetically modified food - Wikipedia

    en.wikipedia.org/wiki/Genetically_modified_food

    This is an accepted version of this page This is the latest accepted revision, reviewed on 28 January 2025. Foods produced from organisms that have had changes introduced into their DNA Part of a series on Genetic engineering Genetically modified organisms Bacteria Viruses Animals Mammals Fish Insects Plants Maize/corn Rice Soybean Potato History and regulation History Regulation Substantial ...

  6. Virotherapy - Wikipedia

    en.wikipedia.org/wiki/Virotherapy

    Oncolytic virotherapy is not a new idea – as early as the mid 1950s doctors were noticing that cancer patients who suffered a non-related viral infection, or who had been vaccinated recently, showed signs of improvement; [3] this has been largely attributed to the production of interferon and tumour necrosis factors in response to viral infection, but oncolytic viruses are being designed ...

  7. Genetically modified organism - Wikipedia

    en.wikipedia.org/wiki/Genetically_modified_organism

    A genetically modified organism (GMO) is any organism whose genetic material has been altered using genetic engineering techniques.The exact definition of a genetically modified organism and what constitutes genetic engineering varies, with the most common being an organism altered in a way that "does not occur naturally by mating and/or natural recombination". [1]

  8. Gene therapy - Wikipedia

    en.wikipedia.org/wiki/Gene_therapy

    The treatment of glioblastoma multiforme, the malignant brain tumor whose outcome is always fatal, was done using a vector expressing antisense IGF-I RNA (clinical trial approved by NIH protocol no.1602 24 November 1993, [168] and by the FDA in 1994). This therapy also represents the beginning of cancer immunogene therapy, a treatment which ...

  9. Gene targeting - Wikipedia

    en.wikipedia.org/wiki/Gene_targeting

    As mosses are haploid organisms, [3] moss filaments can be directly screened for the target, either by treatment with antibiotics or with PCR. Unique among plants, this procedure for reverse genetics is as efficient as in yeast. [4] Gene targeting has been successfully applied to cattle, sheep, swine and many fungi.

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