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A photo of human embryonic stem cells (the cell colonies in the center). Spindle cells surrounding the stem cell colony are MEFs. Mouse Embryonic Fibroblasts (MEFs) are a type of fibroblast prepared from mouse embryo. MEFs show a spindle shape when cultured in vitro, a typical feature of fibroblasts. The MEF is a limited cell line.
The knocked-out embryonic stem cells from step 4 are inserted into a mouse blastocyst. For this example, we use blastocysts from a grey mouse. For this example, we use blastocysts from a grey mouse. The blastocysts now contain two types of stem cells: the original ones (from the grey mouse), and the knocked-out cells (from the white mouse).
The primary mouse embryonic fibroblast cells were transferred (the "T") every 3 days (the first "3"), and inoculated at the rigid density of 3 × 10 5 cells per 20 cm 2 dish (the second "3") continuously. [2] The spontaneously immortalized cells with stable growth rate were established after 20 to 30 generations in culture, and then named '3T3 ...
In view of the potential clinical applications of stem cell-derived tissues or primary epithelial cells, the use of human fibroblasts as an alternative to MEF feeders has been studied. [14] Whereas the fibroblasts are usually used to maintain pluripotency of the stem cells, they can also be used to facilitate development of the stem cells into ...
EMAGE (e-Mouse Atlas of Gene Expression [note 1]) is an online biological database of gene expression data in the developing mouse (Mus musculus) embryo. [1] [2] [3] The data held in EMAGE is spatially annotated to a framework of 3D mouse embryo models produced by EMAP (e-Mouse Atlas Project).
However, three separate groups were able to find mouse embryonic fibroblast (MEF)-derived iPS cells that could be injected into tetraploid blastocysts and resulted in the live birth of mice derived entirely from iPS cells, thus ending the debate over the equivalence of embryonic stem cells (ESCs) and iPS with regard to pluripotency. [41]
The genetically modified mouse in which a gene affecting hair growth has been knocked out (left) shown next to a normal lab mouse. A genetically modified mouse, genetically engineered mouse model (GEMM) [1] or transgenic mouse is a mouse (Mus musculus) that has had its genome altered through the use of genetic engineering techniques.
There are several examples of immortalised cell lines, each with different properties. Most immortalised cell lines are classified by the cell type they originated from or are most similar to biologically 3T3 cells – a mouse fibroblast cell line derived from a spontaneous mutation in cultured mouse embryo tissue.